Estrogen receptor-1 (Esr1) and -2 (Esr2) regulate the severity of clinical experimental allergic encephalomyelitis in male mice

Magdalena Polanczyk, Srikanth Yellayi, Alex Zamora, Sandhya Subramanian, Micah Tovey, Arthur Vandenbark, Halina Offner, James F. Zachary, Parley D. Fillmore, Elizabeth P. Blankenhorn, Jan Åke Gustafsson, Cory Teuscher

Research output: Contribution to journalArticle

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Abstract

Estrogens and estrogen-receptor signaling function in establishing and regulating the female immune system and it is becoming increasingly evident that they may play a similar role in males. We report that B10.PL/SnJ male mice with a disrupted estrogen receptor-1 (α) gene (Esr1-/-) develop less severe clinical experimental allergic encephalomyelitis (EAE) compared to either Esr1+/- or wild-type (Esr1+/+) controls when immunized with myelin basic protein peptide Ac1-11 (MBP Ac1-11). In contrast, the disease course in B10.PL/SnJ male mice with a disrupted estrogen receptor-2 (β) gene (Esr2-/-) does not differ from that of wild-type (Esr2+/+) mice. However, Esr2 +/- mice do develop more severe clinical disease with an earlier onset indicating that heterosis at Esr2 plays a significant role in regulating EAE in males. No significant differences in central nervous system histopathology or MBPAc1-11-specific T-cell responses as assessed by proliferation and interleukin-2 production were observed as a function of either Esr1 or Esr2 genotype. An analysis of cytokine/chemokine secretion by MBPAc1-11-specific T cells revealed unique Esr1 and Esr2 genotype-dependent regulation. Interferon-γ secretion was found to be negatively regulated by Esr1 whereas interleukin-6 and tumor necrosis factor-α secretion exhibited classical Esr2 gene dose responses. Interestingly, MCP-1 displayed distinctively unique patterns of genotype-dependent regulation by Esr1 and Esr2. The contribution of the hematopoietic and nonhematopoietic cellular compartments associated with the heterotic effect at Esr2 in regulating the severity of clinical EAE was identified using reciprocal hematopoietic radiation bone marrow chimeras generated between male wild-type and Esr2+/- mice. Wild-type → Esr2+/- mice exhibited EAE equivalent in severity to that seen in Esr2+/- → Esr2+/- control constructs; both of which were more severe than the clinical signs observed in Esr2+/- → wild-type and wild-type → wild-type mice. These results indicate that the heterotic effect at Esr2 is a function of the nonhematopoietic compartment.

Original languageEnglish (US)
Pages (from-to)1915-1924
Number of pages10
JournalAmerican Journal of Pathology
Volume164
Issue number6
StatePublished - Jun 2004

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Estrogen Receptor beta
Autoimmune Experimental Encephalomyelitis
Estrogen Receptor alpha
Genotype
Genes
Hybrid Vigor
T-Lymphocytes
Chemokines
Estrogen Receptors
Interferons
Interleukin-2
Immune System
Interleukin-6
Estrogens
Central Nervous System
Tumor Necrosis Factor-alpha
Bone Marrow
Radiation
Cytokines
Peptides

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Polanczyk, M., Yellayi, S., Zamora, A., Subramanian, S., Tovey, M., Vandenbark, A., ... Teuscher, C. (2004). Estrogen receptor-1 (Esr1) and -2 (Esr2) regulate the severity of clinical experimental allergic encephalomyelitis in male mice. American Journal of Pathology, 164(6), 1915-1924.

Estrogen receptor-1 (Esr1) and -2 (Esr2) regulate the severity of clinical experimental allergic encephalomyelitis in male mice. / Polanczyk, Magdalena; Yellayi, Srikanth; Zamora, Alex; Subramanian, Sandhya; Tovey, Micah; Vandenbark, Arthur; Offner, Halina; Zachary, James F.; Fillmore, Parley D.; Blankenhorn, Elizabeth P.; Gustafsson, Jan Åke; Teuscher, Cory.

In: American Journal of Pathology, Vol. 164, No. 6, 06.2004, p. 1915-1924.

Research output: Contribution to journalArticle

Polanczyk, M, Yellayi, S, Zamora, A, Subramanian, S, Tovey, M, Vandenbark, A, Offner, H, Zachary, JF, Fillmore, PD, Blankenhorn, EP, Gustafsson, JÅ & Teuscher, C 2004, 'Estrogen receptor-1 (Esr1) and -2 (Esr2) regulate the severity of clinical experimental allergic encephalomyelitis in male mice', American Journal of Pathology, vol. 164, no. 6, pp. 1915-1924.
Polanczyk, Magdalena ; Yellayi, Srikanth ; Zamora, Alex ; Subramanian, Sandhya ; Tovey, Micah ; Vandenbark, Arthur ; Offner, Halina ; Zachary, James F. ; Fillmore, Parley D. ; Blankenhorn, Elizabeth P. ; Gustafsson, Jan Åke ; Teuscher, Cory. / Estrogen receptor-1 (Esr1) and -2 (Esr2) regulate the severity of clinical experimental allergic encephalomyelitis in male mice. In: American Journal of Pathology. 2004 ; Vol. 164, No. 6. pp. 1915-1924.
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abstract = "Estrogens and estrogen-receptor signaling function in establishing and regulating the female immune system and it is becoming increasingly evident that they may play a similar role in males. We report that B10.PL/SnJ male mice with a disrupted estrogen receptor-1 (α) gene (Esr1-/-) develop less severe clinical experimental allergic encephalomyelitis (EAE) compared to either Esr1+/- or wild-type (Esr1+/+) controls when immunized with myelin basic protein peptide Ac1-11 (MBP Ac1-11). In contrast, the disease course in B10.PL/SnJ male mice with a disrupted estrogen receptor-2 (β) gene (Esr2-/-) does not differ from that of wild-type (Esr2+/+) mice. However, Esr2 +/- mice do develop more severe clinical disease with an earlier onset indicating that heterosis at Esr2 plays a significant role in regulating EAE in males. No significant differences in central nervous system histopathology or MBPAc1-11-specific T-cell responses as assessed by proliferation and interleukin-2 production were observed as a function of either Esr1 or Esr2 genotype. An analysis of cytokine/chemokine secretion by MBPAc1-11-specific T cells revealed unique Esr1 and Esr2 genotype-dependent regulation. Interferon-γ secretion was found to be negatively regulated by Esr1 whereas interleukin-6 and tumor necrosis factor-α secretion exhibited classical Esr2 gene dose responses. Interestingly, MCP-1 displayed distinctively unique patterns of genotype-dependent regulation by Esr1 and Esr2. The contribution of the hematopoietic and nonhematopoietic cellular compartments associated with the heterotic effect at Esr2 in regulating the severity of clinical EAE was identified using reciprocal hematopoietic radiation bone marrow chimeras generated between male wild-type and Esr2+/- mice. Wild-type → Esr2+/- mice exhibited EAE equivalent in severity to that seen in Esr2+/- → Esr2+/- control constructs; both of which were more severe than the clinical signs observed in Esr2+/- → wild-type and wild-type → wild-type mice. These results indicate that the heterotic effect at Esr2 is a function of the nonhematopoietic compartment.",
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