Estrogen receptor β, but not estrogen receptor α, is present in the vascular endothelium of the human and nonhuman primate endometrium

Hilary O D Critchley, Robert M. Brenner, Teresa A. Henderson, Karin Williams, Nihar R. Nayak, Ov Slayden, Michael R. Millar, Philippa T K Saunders

    Research output: Contribution to journalArticle

    188 Citations (Scopus)

    Abstract

    Estrogen action is dependent upon the presence of specific ligand-activated receptors in target tissues. The aim of the present experiments was to compare the spatial and temporal pattern of expression of estrogen receptor β (ERβ) with that of ERα in full thickness endometrial samples (from the superficial to the basal zone) obtained from both women and rhesus macaques. Immunohistochemical localization with specific antibodies revealed that ERα and ERβ were both expressed in nuclei of the glands and stroma. Consistent with previous studies, expression of ERα declined in the glands and stroma of the functionalis during the secretory phase. The luminal epithelium also displayed positive immunoreactivity for ERβ. Expression of ERβ declined in glandular cell nuclei, but not stroma, within the functionalis during the late secretory phase. Levels of expression of ERα and ERβ in all cellular compartments remained unchanged in the basalis. Both receptor subtypes were detected on Western blots using proteins extracted from uterine samples obtained throughout the menstrual cycle. There was a striking contrast between the pattern of expression of ERα and ERβ in the vascular endothelium and the perivascular cells surrounding endometrial blood vessels; only ERβ was present in the endothelial cell population, although both forms of ER were expressed in perivascular cells. We conclude that estrogen action(s) within the vascular endothelium in the endometrium may be mediated via direct binding to the ERβ isoform and that these cells could therefore be a target for agonists or antagonists that selectively target the β form of the ER.

    Original languageEnglish (US)
    Pages (from-to)1370-1378
    Number of pages9
    JournalJournal of Clinical Endocrinology and Metabolism
    Volume86
    Issue number3
    DOIs
    StatePublished - 2001

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    Vascular Endothelium
    Endometrium
    Estrogen Receptors
    Primates
    Estrogens
    Endothelial cells
    Blood vessels
    Menstrual Cycle
    Macaca mulatta
    Cell Nucleus
    Blood Vessels

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Estrogen receptor β, but not estrogen receptor α, is present in the vascular endothelium of the human and nonhuman primate endometrium. / Critchley, Hilary O D; Brenner, Robert M.; Henderson, Teresa A.; Williams, Karin; Nayak, Nihar R.; Slayden, Ov; Millar, Michael R.; Saunders, Philippa T K.

    In: Journal of Clinical Endocrinology and Metabolism, Vol. 86, No. 3, 2001, p. 1370-1378.

    Research output: Contribution to journalArticle

    Critchley, Hilary O D ; Brenner, Robert M. ; Henderson, Teresa A. ; Williams, Karin ; Nayak, Nihar R. ; Slayden, Ov ; Millar, Michael R. ; Saunders, Philippa T K. / Estrogen receptor β, but not estrogen receptor α, is present in the vascular endothelium of the human and nonhuman primate endometrium. In: Journal of Clinical Endocrinology and Metabolism. 2001 ; Vol. 86, No. 3. pp. 1370-1378.
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    abstract = "Estrogen action is dependent upon the presence of specific ligand-activated receptors in target tissues. The aim of the present experiments was to compare the spatial and temporal pattern of expression of estrogen receptor β (ERβ) with that of ERα in full thickness endometrial samples (from the superficial to the basal zone) obtained from both women and rhesus macaques. Immunohistochemical localization with specific antibodies revealed that ERα and ERβ were both expressed in nuclei of the glands and stroma. Consistent with previous studies, expression of ERα declined in the glands and stroma of the functionalis during the secretory phase. The luminal epithelium also displayed positive immunoreactivity for ERβ. Expression of ERβ declined in glandular cell nuclei, but not stroma, within the functionalis during the late secretory phase. Levels of expression of ERα and ERβ in all cellular compartments remained unchanged in the basalis. Both receptor subtypes were detected on Western blots using proteins extracted from uterine samples obtained throughout the menstrual cycle. There was a striking contrast between the pattern of expression of ERα and ERβ in the vascular endothelium and the perivascular cells surrounding endometrial blood vessels; only ERβ was present in the endothelial cell population, although both forms of ER were expressed in perivascular cells. We conclude that estrogen action(s) within the vascular endothelium in the endometrium may be mediated via direct binding to the ERβ isoform and that these cells could therefore be a target for agonists or antagonists that selectively target the β form of the ER.",
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    T1 - Estrogen receptor β, but not estrogen receptor α, is present in the vascular endothelium of the human and nonhuman primate endometrium

    AU - Critchley, Hilary O D

    AU - Brenner, Robert M.

    AU - Henderson, Teresa A.

    AU - Williams, Karin

    AU - Nayak, Nihar R.

    AU - Slayden, Ov

    AU - Millar, Michael R.

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