Estrogen modulation of the α-1-adrenergic response of hypothalamic neurons

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Intracellular recordings were made from 106 arcuate and cell-poor zone (ARC-CPZ) neurons in sagittal slices prepared from intact, ovariectomized and ovariectomized plus estradiol-benzoate-treated female guinea pigs, and the effects of norepinephrine (NE), the α1-agonist methoxamine (MX) and the β-agonist isoproterenol were tested. Either bath application or pressure application of 2-100 μM NE reversibly hyperpolarized and inhibited the spontaneous firing of the majority (57%, n = 60) of ARC-CPZ neurons. Isoproterenol also inhibited the majority (75%) of the ARC-CPZ neurons which it was tested on. In addition, 2-100 μM NE depolarized and/or increased the spontaneous activity of 20% (n = 21) of ARC-CPZ neurons, and some of these (n = 8) exhibited bursting activity. Similar doses of MX mimicked the NE excitation (depolarization and/or increased firing) in 48% (n = 14) of the ARC-CPZ neurons tested. Based on the serum levels of 17β-estradiol, the three groups of females were divided into high (>30 pg/ml) and low (1-adrenergic stimulation.

Original languageEnglish (US)
Pages (from-to)51-58
Number of pages8
JournalNeuroendocrinology
Volume50
Issue number1
StatePublished - 1989

Fingerprint

Adrenergic Agents
Estrogens
Norepinephrine
Neurons
Methoxamine
Isoproterenol
Baths
Estradiol
Guinea Pigs
Pressure
Serum

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Estrogen modulation of the α-1-adrenergic response of hypothalamic neurons. / Condon, T. P.; Ronnekleiv, Oline; Kelly, Martin.

In: Neuroendocrinology, Vol. 50, No. 1, 1989, p. 51-58.

Research output: Contribution to journalArticle

@article{c14fc5ce419d4d3f881104f21ecaf15f,
title = "Estrogen modulation of the α-1-adrenergic response of hypothalamic neurons",
abstract = "Intracellular recordings were made from 106 arcuate and cell-poor zone (ARC-CPZ) neurons in sagittal slices prepared from intact, ovariectomized and ovariectomized plus estradiol-benzoate-treated female guinea pigs, and the effects of norepinephrine (NE), the α1-agonist methoxamine (MX) and the β-agonist isoproterenol were tested. Either bath application or pressure application of 2-100 μM NE reversibly hyperpolarized and inhibited the spontaneous firing of the majority (57{\%}, n = 60) of ARC-CPZ neurons. Isoproterenol also inhibited the majority (75{\%}) of the ARC-CPZ neurons which it was tested on. In addition, 2-100 μM NE depolarized and/or increased the spontaneous activity of 20{\%} (n = 21) of ARC-CPZ neurons, and some of these (n = 8) exhibited bursting activity. Similar doses of MX mimicked the NE excitation (depolarization and/or increased firing) in 48{\%} (n = 14) of the ARC-CPZ neurons tested. Based on the serum levels of 17β-estradiol, the three groups of females were divided into high (>30 pg/ml) and low (1-adrenergic stimulation.",
author = "Condon, {T. P.} and Oline Ronnekleiv and Martin Kelly",
year = "1989",
language = "English (US)",
volume = "50",
pages = "51--58",
journal = "Neuroendocrinology",
issn = "0028-3835",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Estrogen modulation of the α-1-adrenergic response of hypothalamic neurons

AU - Condon, T. P.

AU - Ronnekleiv, Oline

AU - Kelly, Martin

PY - 1989

Y1 - 1989

N2 - Intracellular recordings were made from 106 arcuate and cell-poor zone (ARC-CPZ) neurons in sagittal slices prepared from intact, ovariectomized and ovariectomized plus estradiol-benzoate-treated female guinea pigs, and the effects of norepinephrine (NE), the α1-agonist methoxamine (MX) and the β-agonist isoproterenol were tested. Either bath application or pressure application of 2-100 μM NE reversibly hyperpolarized and inhibited the spontaneous firing of the majority (57%, n = 60) of ARC-CPZ neurons. Isoproterenol also inhibited the majority (75%) of the ARC-CPZ neurons which it was tested on. In addition, 2-100 μM NE depolarized and/or increased the spontaneous activity of 20% (n = 21) of ARC-CPZ neurons, and some of these (n = 8) exhibited bursting activity. Similar doses of MX mimicked the NE excitation (depolarization and/or increased firing) in 48% (n = 14) of the ARC-CPZ neurons tested. Based on the serum levels of 17β-estradiol, the three groups of females were divided into high (>30 pg/ml) and low (1-adrenergic stimulation.

AB - Intracellular recordings were made from 106 arcuate and cell-poor zone (ARC-CPZ) neurons in sagittal slices prepared from intact, ovariectomized and ovariectomized plus estradiol-benzoate-treated female guinea pigs, and the effects of norepinephrine (NE), the α1-agonist methoxamine (MX) and the β-agonist isoproterenol were tested. Either bath application or pressure application of 2-100 μM NE reversibly hyperpolarized and inhibited the spontaneous firing of the majority (57%, n = 60) of ARC-CPZ neurons. Isoproterenol also inhibited the majority (75%) of the ARC-CPZ neurons which it was tested on. In addition, 2-100 μM NE depolarized and/or increased the spontaneous activity of 20% (n = 21) of ARC-CPZ neurons, and some of these (n = 8) exhibited bursting activity. Similar doses of MX mimicked the NE excitation (depolarization and/or increased firing) in 48% (n = 14) of the ARC-CPZ neurons tested. Based on the serum levels of 17β-estradiol, the three groups of females were divided into high (>30 pg/ml) and low (1-adrenergic stimulation.

UR - http://www.scopus.com/inward/record.url?scp=0024309540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024309540&partnerID=8YFLogxK

M3 - Article

VL - 50

SP - 51

EP - 58

JO - Neuroendocrinology

JF - Neuroendocrinology

SN - 0028-3835

IS - 1

ER -