Estrogen inhibits systemic T cell expression of TNF-α and recruitment of TNF-α+ T cells and macrophages into the CNS of mice developing experimental encephalomyelitis

Atsushi Ito, Abigail C. Buenafe, Agata Matejuk, Alex Zamora, Marc Silverman, Jami Dwyer, Arthur Vandenbark, Halina Offner

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Estrogen treatment has been found to have suppressive activity in several models of autoimmunity. To investigate the mechanism of 17β-estradiol (E2) suppression of experimental autoimmune encephalomyelitis, we evaluated E2 effects on TNF-α expression in the central nervous system (CNS) and spleen of C57BL/6 mice immunized with MOG 35-55/CFA. Kinetic analysis demonstrated that E2 treatment drastically decreased the recruitment of total inflammatory cells as well as TNF-α+ macrophages and T cells into the CNS at disease onset. In contrast, E2 had only moderate effects on the relatively high constitutive TNF-α expression by resident CNS microglial cells. E2 treatment also had profound inhibitory effects on expression of TNF-α by splenic CD4+ T cells, including those responsive to MOG 35-55 peptide. We propose that the mechanism of E2 protection may involve both systemic inhibition of TNF-α expression and local (CNS) recruitment of inflammatory cells, with modest effects on TNF-α expression by resident CNS microglial cells.

Original languageEnglish (US)
Pages (from-to)275-282
Number of pages8
JournalClinical Immunology
Volume102
Issue number3
DOIs
StatePublished - 2002

Fingerprint

Neurologic Mutant Mice
Encephalomyelitis
Estrogens
Central Nervous System
Macrophages
T-Lymphocytes
Autoimmune Experimental Encephalomyelitis
Central Nervous System Diseases
Autoimmunity
Inbred C57BL Mouse
Estradiol
Spleen
Peptides

Keywords

  • 17β-estradiol
  • CNS
  • EAE
  • Macrophages
  • T cells
  • TNF-α

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Estrogen inhibits systemic T cell expression of TNF-α and recruitment of TNF-α+ T cells and macrophages into the CNS of mice developing experimental encephalomyelitis. / Ito, Atsushi; Buenafe, Abigail C.; Matejuk, Agata; Zamora, Alex; Silverman, Marc; Dwyer, Jami; Vandenbark, Arthur; Offner, Halina.

In: Clinical Immunology, Vol. 102, No. 3, 2002, p. 275-282.

Research output: Contribution to journalArticle

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