Abstract
Estrogen treatment has been found to have suppressive activity in several models of autoimmunity. To investigate the mechanism of 17β-estradiol (E2) suppression of experimental autoimmune encephalomyelitis, we evaluated E2 effects on TNF-α expression in the central nervous system (CNS) and spleen of C57BL/6 mice immunized with MOG 35-55/CFA. Kinetic analysis demonstrated that E2 treatment drastically decreased the recruitment of total inflammatory cells as well as TNF-α+ macrophages and T cells into the CNS at disease onset. In contrast, E2 had only moderate effects on the relatively high constitutive TNF-α expression by resident CNS microglial cells. E2 treatment also had profound inhibitory effects on expression of TNF-α by splenic CD4+ T cells, including those responsive to MOG 35-55 peptide. We propose that the mechanism of E2 protection may involve both systemic inhibition of TNF-α expression and local (CNS) recruitment of inflammatory cells, with modest effects on TNF-α expression by resident CNS microglial cells.
Original language | English (US) |
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Pages (from-to) | 275-282 |
Number of pages | 8 |
Journal | Clinical Immunology |
Volume | 102 |
Issue number | 3 |
DOIs | |
State | Published - 2002 |
Keywords
- 17β-estradiol
- CNS
- EAE
- Macrophages
- T cells
- TNF-α
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology