Estrogen inhibition of EAE involves effects on dendritic cell function

Hong Yan Liu, Abigail C. Buenafe, Agata Matejuk, Atsushi Ito, Alex Zamora, Jami Dwyer, Arthur Vandenbark, Halina Offner

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Estrogen has been found to have suppressive effects on the induction of experimental autoimmune encephalomyelitis (EAE), an animal model for the human disease multiple sclerosis. We have investigated the effects of 17β-estradiol (E2) treatment on dendritic cells (DCs) in two different mouse models of EAE. The frequency of CD11b+/CD11c+ DCs was significantly decreased in the brain of mice protected from EAE induction by E2 treatment. In addition, the frequency of CD11c+/CD8α+ DCs producing tumor necrosis factor (TNF)α and interferon (IFN)γ in the spleen of E2-treated mice was dramatically decreased compared to that in control mice with EAE, demonstrating an effect of E2 on DC function. In order to examine E2 effects on DCs in more detail, splenic DCs were cultured in the presence of granulocytemacrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4to promote maturation. E2 pretreatment was found to suppress the ability of cultured DCs bearing a mature phenotype to present Ag to myelin basic protein (MBP)-specific T cells. Analysis of cytokine production demonstrated that E2 decreased TNFα, IFNγ and IL-12 production in mature DCs. In addition, MBP-specific T cells cocultured with E2-pretreated mature DCs in the presence of antigen demonstrated a shift towards production of Th2 cytokines IL-4 and IL-10 and a concomitant decrease in the production of Th1 cytokines TNFα and IFNγ. Thus, E2 treatment appears to have multiple effects on the DC population, which may contribute to a down-regulation or block in the activation of Th1 cells involved in the induction of EAE.

Original languageEnglish (US)
Pages (from-to)238-248
Number of pages11
JournalJournal of Neuroscience Research
Volume70
Issue number2
DOIs
StatePublished - Oct 15 2002

Fingerprint

Autoimmune Experimental Encephalomyelitis
Dendritic Cells
Estrogens
Interferons
Myelin Basic Protein
Tumor Necrosis Factor-alpha
Cytokines
T-Lymphocytes
Animal Disease Models
Colony-Stimulating Factors
Th1 Cells
Interleukins
Interleukin-12
Interleukin-4
Interleukin-10
Multiple Sclerosis
Estradiol
Cultured Cells
Down-Regulation
Spleen

Keywords

  • Dendritic cells
  • EAE
  • Estrogen

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Estrogen inhibition of EAE involves effects on dendritic cell function. / Liu, Hong Yan; Buenafe, Abigail C.; Matejuk, Agata; Ito, Atsushi; Zamora, Alex; Dwyer, Jami; Vandenbark, Arthur; Offner, Halina.

In: Journal of Neuroscience Research, Vol. 70, No. 2, 15.10.2002, p. 238-248.

Research output: Contribution to journalArticle

Liu HY, Buenafe AC, Matejuk A, Ito A, Zamora A, Dwyer J et al. Estrogen inhibition of EAE involves effects on dendritic cell function. Journal of Neuroscience Research. 2002 Oct 15;70(2):238-248. https://doi.org/10.1002/jnr.10409
Liu, Hong Yan ; Buenafe, Abigail C. ; Matejuk, Agata ; Ito, Atsushi ; Zamora, Alex ; Dwyer, Jami ; Vandenbark, Arthur ; Offner, Halina. / Estrogen inhibition of EAE involves effects on dendritic cell function. In: Journal of Neuroscience Research. 2002 ; Vol. 70, No. 2. pp. 238-248.
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