Estradiol increases α7 nicotinic receptor in serotonergic dorsal raphe and noradrenergic locus coeruleus neurons of macaques

Maria Luisa Centeno, Jessica A. Henderson, K. Y Francis Pau, Cynthia Bethea

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    Acetylcholine, acting on presynaptic nicotinic receptors (nAChRs), modulates the release of neurotransmitters in the brain. The rat dorsal raphe nucleus (DR) and the locus coeruleus (LC) receive cholinergic input and express the α7nAChR. In previous reports, we demonstrated that estradiol (E) administration stimulates DR serotonergic and LC noradrenergic function in the macaque. In addition, it has been reported that E induces the expression of the α7nAChR in rats. We questioned whether E increased the expression of the α7nAChR in the macaque DR and LC. We utilized double immunostaining to study the effect of a simulated preovulatory surge of E on the expression of the α7nAChR in the DR and the LC and to determine whether α7nAChR colocalizes with serotonin and tyrosine hydroxylase (TH) in macaques. There was no difference in the number of α7nAChR-positive neurons between ovariectomized (OVX) controls and OVX animals treated with a silastic capsule containing E (Ecap). However, supplemental infusion of E for 5-30 hours to Ecap animals (Ecap + inf) significantly increased the number of α7nAChR- positive neurons in DR and LC. In addition, supplemental E infusion significantly increased the number of neurons in which α7nAChR colocalized with serotonin and TH. These results constitute an important antecedent for study of the effects of nicotine and ovarian steroid hormones in the physiological functions regulated by the DR and the LC in women.

    Original languageEnglish (US)
    Pages (from-to)489-501
    Number of pages13
    JournalJournal of Comparative Neurology
    Volume497
    Issue number3
    DOIs
    StatePublished - Jul 20 2006

    Fingerprint

    Locus Coeruleus
    Nicotinic Receptors
    Macaca
    Estradiol
    Neurons
    Tyrosine 3-Monooxygenase
    Serotonin
    Presynaptic Receptors
    Nicotine
    Cholinergic Agents
    Acetylcholine
    Capsules
    Neurotransmitter Agents
    Dorsal Raphe Nucleus
    Steroids
    Hormones
    Brain

    Keywords

    • Acetylcholine
    • Nicotinic receptors
    • Noradrenaline
    • Serotonin
    • Steroid hormones

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Estradiol increases α7 nicotinic receptor in serotonergic dorsal raphe and noradrenergic locus coeruleus neurons of macaques. / Centeno, Maria Luisa; Henderson, Jessica A.; Pau, K. Y Francis; Bethea, Cynthia.

    In: Journal of Comparative Neurology, Vol. 497, No. 3, 20.07.2006, p. 489-501.

    Research output: Contribution to journalArticle

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