Estimate of the chloride concentration in a central glutamatergic terminal: A gramicidin perforated-patch study on the calyx of held

Gareth D. Price, Laurence Trussell

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

The function of presynaptic terminals is regulated by intracellular Cl -, the levels of which modify vesicular endocytosis and transmitter refilling and mediate the effects of presynaptic ligand-gated Cl- channels. Nevertheless, the concentration of Cl- in a central nerve terminal is unknown, and it is unclear whether terminals can regulate Cl - independently of the soma. Using perforated-patch recording in a mammalian synapse, we found that terminals accumulate Cl- up to 21mM, between four and five times higher than in their parent cell bodies. Changing [Cl-] did not alter vesicular glutamate content in intact terminals, unlike in vitro experiments. Thus, glutamatergic terminals maintain an elevated Cl- concentration without compromising synaptic transmission.

Original languageEnglish (US)
Pages (from-to)11432-11436
Number of pages5
JournalJournal of Neuroscience
Volume26
Issue number44
DOIs
StatePublished - Nov 1 2006

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Ligand-Gated Ion Channels
Gramicidin
Presynaptic Terminals
Carisoprodol
Endocytosis
Synaptic Transmission
Synapses
Chlorides
Glutamic Acid
Cell Body
In Vitro Techniques

Keywords

  • Auditory
  • Calyx of held
  • Chloride
  • Glycinergic
  • KCC2
  • Perforated-patch

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Estimate of the chloride concentration in a central glutamatergic terminal : A gramicidin perforated-patch study on the calyx of held. / Price, Gareth D.; Trussell, Laurence.

In: Journal of Neuroscience, Vol. 26, No. 44, 01.11.2006, p. 11432-11436.

Research output: Contribution to journalArticle

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