Estimate of the chloride concentration in a central glutamatergic terminal: A gramicidin perforated-patch study on the calyx of held

Gareth D. Price, Laurence O. Trussell

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

The function of presynaptic terminals is regulated by intracellular Cl -, the levels of which modify vesicular endocytosis and transmitter refilling and mediate the effects of presynaptic ligand-gated Cl- channels. Nevertheless, the concentration of Cl- in a central nerve terminal is unknown, and it is unclear whether terminals can regulate Cl - independently of the soma. Using perforated-patch recording in a mammalian synapse, we found that terminals accumulate Cl- up to 21mM, between four and five times higher than in their parent cell bodies. Changing [Cl-] did not alter vesicular glutamate content in intact terminals, unlike in vitro experiments. Thus, glutamatergic terminals maintain an elevated Cl- concentration without compromising synaptic transmission.

Original languageEnglish (US)
Pages (from-to)11432-11436
Number of pages5
JournalJournal of Neuroscience
Volume26
Issue number44
DOIs
StatePublished - Nov 1 2006

Keywords

  • Auditory
  • Calyx of held
  • Chloride
  • Glycinergic
  • KCC2
  • Perforated-patch

ASJC Scopus subject areas

  • Neuroscience(all)

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