Establishment of cell lines of uveal melanoma. Methodology and characteristics

D. M. Albert, M. A. Ruzzo, M. A. McLaughlin, N. L. Robinson, J. L. Craft, J. Epstein

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Six continuous cell lines have been established from choroidal and ciliary body melanomas. These lines have been maintained in culture for at least 100 in vitro population doublings for periods over 1 year. They were established initially using a human diploid fibroblast strain MRC-5 as a feeder layer. Cells were grown in Ham's F-12 medium containing fetal bovine and horse sera and supplemented with glucose, cholera toxin, and epidermal growth factor. Culture doubling times ranged from 72-96 hr; cloning efficiencies ranged from 1-5% in the absence of a feeder layer. Six cell lines were studied in detail by electron microscopy, and all were found to have evidence of melanosomes and/or premelanosomes. The morphology of the cells was characteristic of melanomas as defined by the Callender classification, with cell types ranging from spindle A to epithelioid. Karyotypic studies revealed the presence of only human chromosomes with modal numbers ranging from 48-54 in the different lines.

Original languageEnglish (US)
Pages (from-to)1284-1299
Number of pages16
JournalInvestigative Ophthalmology and Visual Science
Volume25
Issue number11
StatePublished - Dec 1 1984

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Albert, D. M., Ruzzo, M. A., McLaughlin, M. A., Robinson, N. L., Craft, J. L., & Epstein, J. (1984). Establishment of cell lines of uveal melanoma. Methodology and characteristics. Investigative Ophthalmology and Visual Science, 25(11), 1284-1299.