Establishment of cell lines of uveal melanoma. Methodology and characteristics

Daniel Albert, M. A. Ruzzo, M. A. McLaughlin, N. L. Robinson, J. L. Craft, J. Epstein

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Six continuous cell lines have been established from choroidal and ciliary body melanomas. These lines have been maintained in culture for at least 100 in vitro population doublings for periods over 1 year. They were established initially using a human diploid fibroblast strain MRC-5 as a feeder layer. Cells were grown in Ham's F-12 medium containing fetal bovine and horse sera and supplemented with glucose, cholera toxin, and epidermal growth factor. Culture doubling times ranged from 72-96 hr; cloning efficiencies ranged from 1-5% in the absence of a feeder layer. Six cell lines were studied in detail by electron microscopy, and all were found to have evidence of melanosomes and/or premelanosomes. The morphology of the cells was characteristic of melanomas as defined by the Callender classification, with cell types ranging from spindle A to epithelioid. Karyotypic studies revealed the presence of only human chromosomes with modal numbers ranging from 48-54 in the different lines.

Original languageEnglish (US)
Pages (from-to)1284-1299
Number of pages16
JournalInvestigative Ophthalmology and Visual Science
Volume25
Issue number11
StatePublished - Dec 1 1984
Externally publishedYes

Fingerprint

Feeder Cells
Cell Line
Melanoma
Melanosomes
Ciliary Body
Cholera Toxin
Human Chromosomes
Diploidy
Epidermal Growth Factor
Horses
Organism Cloning
Electron Microscopy
Fibroblasts
Glucose
Serum
Population
Uveal melanoma
In Vitro Techniques

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Albert, D., Ruzzo, M. A., McLaughlin, M. A., Robinson, N. L., Craft, J. L., & Epstein, J. (1984). Establishment of cell lines of uveal melanoma. Methodology and characteristics. Investigative Ophthalmology and Visual Science, 25(11), 1284-1299.

Establishment of cell lines of uveal melanoma. Methodology and characteristics. / Albert, Daniel; Ruzzo, M. A.; McLaughlin, M. A.; Robinson, N. L.; Craft, J. L.; Epstein, J.

In: Investigative Ophthalmology and Visual Science, Vol. 25, No. 11, 01.12.1984, p. 1284-1299.

Research output: Contribution to journalArticle

Albert, D, Ruzzo, MA, McLaughlin, MA, Robinson, NL, Craft, JL & Epstein, J 1984, 'Establishment of cell lines of uveal melanoma. Methodology and characteristics', Investigative Ophthalmology and Visual Science, vol. 25, no. 11, pp. 1284-1299.
Albert D, Ruzzo MA, McLaughlin MA, Robinson NL, Craft JL, Epstein J. Establishment of cell lines of uveal melanoma. Methodology and characteristics. Investigative Ophthalmology and Visual Science. 1984 Dec 1;25(11):1284-1299.
Albert, Daniel ; Ruzzo, M. A. ; McLaughlin, M. A. ; Robinson, N. L. ; Craft, J. L. ; Epstein, J. / Establishment of cell lines of uveal melanoma. Methodology and characteristics. In: Investigative Ophthalmology and Visual Science. 1984 ; Vol. 25, No. 11. pp. 1284-1299.
@article{6f8a3e3668494cb982daab4f44910d15,
title = "Establishment of cell lines of uveal melanoma. Methodology and characteristics",
abstract = "Six continuous cell lines have been established from choroidal and ciliary body melanomas. These lines have been maintained in culture for at least 100 in vitro population doublings for periods over 1 year. They were established initially using a human diploid fibroblast strain MRC-5 as a feeder layer. Cells were grown in Ham's F-12 medium containing fetal bovine and horse sera and supplemented with glucose, cholera toxin, and epidermal growth factor. Culture doubling times ranged from 72-96 hr; cloning efficiencies ranged from 1-5{\%} in the absence of a feeder layer. Six cell lines were studied in detail by electron microscopy, and all were found to have evidence of melanosomes and/or premelanosomes. The morphology of the cells was characteristic of melanomas as defined by the Callender classification, with cell types ranging from spindle A to epithelioid. Karyotypic studies revealed the presence of only human chromosomes with modal numbers ranging from 48-54 in the different lines.",
author = "Daniel Albert and Ruzzo, {M. A.} and McLaughlin, {M. A.} and Robinson, {N. L.} and Craft, {J. L.} and J. Epstein",
year = "1984",
month = "12",
day = "1",
language = "English (US)",
volume = "25",
pages = "1284--1299",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "11",

}

TY - JOUR

T1 - Establishment of cell lines of uveal melanoma. Methodology and characteristics

AU - Albert, Daniel

AU - Ruzzo, M. A.

AU - McLaughlin, M. A.

AU - Robinson, N. L.

AU - Craft, J. L.

AU - Epstein, J.

PY - 1984/12/1

Y1 - 1984/12/1

N2 - Six continuous cell lines have been established from choroidal and ciliary body melanomas. These lines have been maintained in culture for at least 100 in vitro population doublings for periods over 1 year. They were established initially using a human diploid fibroblast strain MRC-5 as a feeder layer. Cells were grown in Ham's F-12 medium containing fetal bovine and horse sera and supplemented with glucose, cholera toxin, and epidermal growth factor. Culture doubling times ranged from 72-96 hr; cloning efficiencies ranged from 1-5% in the absence of a feeder layer. Six cell lines were studied in detail by electron microscopy, and all were found to have evidence of melanosomes and/or premelanosomes. The morphology of the cells was characteristic of melanomas as defined by the Callender classification, with cell types ranging from spindle A to epithelioid. Karyotypic studies revealed the presence of only human chromosomes with modal numbers ranging from 48-54 in the different lines.

AB - Six continuous cell lines have been established from choroidal and ciliary body melanomas. These lines have been maintained in culture for at least 100 in vitro population doublings for periods over 1 year. They were established initially using a human diploid fibroblast strain MRC-5 as a feeder layer. Cells were grown in Ham's F-12 medium containing fetal bovine and horse sera and supplemented with glucose, cholera toxin, and epidermal growth factor. Culture doubling times ranged from 72-96 hr; cloning efficiencies ranged from 1-5% in the absence of a feeder layer. Six cell lines were studied in detail by electron microscopy, and all were found to have evidence of melanosomes and/or premelanosomes. The morphology of the cells was characteristic of melanomas as defined by the Callender classification, with cell types ranging from spindle A to epithelioid. Karyotypic studies revealed the presence of only human chromosomes with modal numbers ranging from 48-54 in the different lines.

UR - http://www.scopus.com/inward/record.url?scp=0021703467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021703467&partnerID=8YFLogxK

M3 - Article

C2 - 6386741

AN - SCOPUS:0021703467

VL - 25

SP - 1284

EP - 1299

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 11

ER -