Escherichia coli tRNA4Arg(UCU) induces a constrained conformation of the crucial Ω-loop of arginyl-tRNA synthetase

Yong Neng Yao, Qing-Shuo Zhang, Xian Zhong Yan, Guang Zhu, En Duo Wang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Previous investigations show that tRNAArg-induced conformational changes of arginyl-tRNA synthetase (ArgRS) Ω-loop region (Escherichia coli (E. coli), Ala451-Ala457) may contribute to the productive conformation of the enzyme catalytic core, and E. coli tRNA2 Arg(ICG)-bound and -free conformations of the Ω-loop exchange at an intermediate rate on NMR timescale. Herein, we report that E. coli ArgRS catalyzes tRNA2Arg(ICG) and tRNA4 Arg(UCU) with similar efficiencies. However, 19F NMR spectroscopy of 4-fluorotryptophan-labeled E. coli ArgRS reveals that the tRNA4Arg(UCU)-bound and -free conformations of the Ω-loop region interconvert very slowly and the lifetime of bound conformation is much longer than 0.33ms. Therefore, tRNA4 Arg(UCU) differs from tRNA2Arg(ICG) in the conformation-exchanging rate of the Ω-loop. Comparative structure model of E. coli ArgRS is presented to rationalize these 19F NMR data. Our 19F NMR and catalytic assay results suggest that the tRNA Arg-induced conformational changes of Ω-loop little contribute to the productive conformation of ArgRS catalytic core.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume313
Issue number1
DOIs
StatePublished - Jan 2 2004
Externally publishedYes

Fingerprint

Arginine-tRNA Ligase
Escherichia coli
Conformations
RNA, Transfer, Arg
Nuclear magnetic resonance
Catalytic Domain
Model structures
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance spectroscopy
Assays
Enzymes

Keywords

  • Aminoacyl-tRNA synthetase
  • Arginine
  • ArgRS
  • argU
  • Comparative modeling
  • tRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Escherichia coli tRNA4Arg(UCU) induces a constrained conformation of the crucial Ω-loop of arginyl-tRNA synthetase. / Yao, Yong Neng; Zhang, Qing-Shuo; Yan, Xian Zhong; Zhu, Guang; Wang, En Duo.

In: Biochemical and Biophysical Research Communications, Vol. 313, No. 1, 02.01.2004, p. 129-134.

Research output: Contribution to journalArticle

Yao, Yong Neng ; Zhang, Qing-Shuo ; Yan, Xian Zhong ; Zhu, Guang ; Wang, En Duo. / Escherichia coli tRNA4Arg(UCU) induces a constrained conformation of the crucial Ω-loop of arginyl-tRNA synthetase. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 313, No. 1. pp. 129-134.
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