TY - JOUR
T1 - Erythroid-specific activation and derepression of the chick β-globin promoter in vitro
AU - Emerson, Beverly M.
AU - Nickol, Joanne M.
AU - Fong, Timothy C.
N1 - Funding Information:
We wish to thank Dr. Katherine Jones for her generous gift of purified Spl and CTF, advice on DNA affinity chromatography, a critical reading of the manuscript, and, most of all, her friendship and encouragement throughout the course of this work. This project was funded by a grant from the National Institutes of Health (GM38760). This paper is dedicated to the loving memory of Marie Emerson, 1920-1989.
PY - 1989/6/30
Y1 - 1989/6/30
N2 - Transcriptionally active extracts were prepared from chick red cells isolated at different stages of development. The template activity of cloned β-globin genes is highest in extracts from definitive red cells, where the endogenous gene is normally expressed, and lowest in extracts from primitive red cells or nonery-throid tissues. This system has been used to identify regulatory elements and to assign functions to the proteins that bind within the β-globin promoter. Regulation of expression is achieved, in part, by factors whose composition changes during red cell development. Two proteins, PAL and CON, bind at adjacent sites but have opposite effects on transcription in vitro. Levels of PAL, a potent repressor, are highest in mature red cells while those of CON, an activator, are highest in actively transcribing red cells. The effect of PAL can be overcome by blocking its binding site with a protein having a similar recognition sequence but a dissimilar function.
AB - Transcriptionally active extracts were prepared from chick red cells isolated at different stages of development. The template activity of cloned β-globin genes is highest in extracts from definitive red cells, where the endogenous gene is normally expressed, and lowest in extracts from primitive red cells or nonery-throid tissues. This system has been used to identify regulatory elements and to assign functions to the proteins that bind within the β-globin promoter. Regulation of expression is achieved, in part, by factors whose composition changes during red cell development. Two proteins, PAL and CON, bind at adjacent sites but have opposite effects on transcription in vitro. Levels of PAL, a potent repressor, are highest in mature red cells while those of CON, an activator, are highest in actively transcribing red cells. The effect of PAL can be overcome by blocking its binding site with a protein having a similar recognition sequence but a dissimilar function.
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U2 - 10.1016/0092-8674(89)90056-1
DO - 10.1016/0092-8674(89)90056-1
M3 - Article
C2 - 2736626
AN - SCOPUS:0024392447
SN - 0092-8674
VL - 57
SP - 1189
EP - 1200
JO - Cell
JF - Cell
IS - 7
ER -