Erratum: Mesenchymal stromal cell-derived extracellular vesicles promote myeloid-biased multipotent hematopoietic progenitor expansion via Toll-like receptor engagement (The Journal of Biological Chemistry (2016) 291 (24607-24617) DOI: 10.1074/jbc.A116.745653)

Natalya A. Goloviznina, Santhosh Chakkaramakkil Verghese, Young Me Yoon, Oleh Taratula, Daniel Marks, Peter Kurre

Research output: Contribution to journalComment/debate

1 Citation (Scopus)

Abstract

There were several errors in the original figure legend to Fig. 4. The figure legend should be replaced with the following. FIGURE 4. MSC-derived EVs activate NF-κB and Notch signaling to promote HSPC differentiation. A, diagrammatic representation of the lentiviral vector design used to generate the NF-κB enhancer-luciferase reporter cell line. Stably transduced and sorted SIM-A9 microglial cells were used for EV exposure followed by luciferase assay. The SIM-A9-NF-κB-Luc cells were exposed with MSC EVs and incubated for 48 h followed by luciferase assay. Non-treated SIM-A9-NF-κB-Luc and LPS-treated SIM-A9-NF-κB-Luc cells were used as negative and positive controls, respectively. Luciferase activity was measured as relative fluorescence units (RFU). Data represent n = 4 independent experiments; error bars depict S.D. All p values have been calculated using two-tailed Student's t test. B, quantitative mRNA expression of NF-κB and Notch-1 signaling and downstream targets involved in cell proliferation in MSC EV-exposed HSPCs. Data represent n = 3 independent experiments; error bars are S.D. C, we next tested a candidate panel of canonical TLR4 responsive cytokines for both transcriptional activation and secretion, either after EV or S100 exposure. Transcriptional analysis of EV-exposed HSPCs reveals an up-regulation of several known TLR4-responsive cytokine genes (IL6, TNFa, Stat1, and EGFR) in WT but not MyD88-/- HSPCs. Data represent n = 3 independent experiments; error bars are S.D. D, TLR4 signaling was specifically inhibited in WT MSC-EV-exposed HSPCs using TAK-242. Transcriptional analysis of EV-exposed HSPCs with and without TLR4 inhibitor showed down-regulation of the same TLR-responsive genes described above. Data represent n = 2 independent experiments; error bars represent S.D. E, cytokines released by HSPCs after 48 h co-culture, with MSC EVs compared with S100, were measured using a Luminex assay, whereby error bars are S.D. and p values are an indication of the variance across three technical replicates in this screening panel.

Original languageEnglish (US)
Pages (from-to)3541
Number of pages1
JournalJournal of Biological Chemistry
Volume292
Issue number8
DOIs
StatePublished - Feb 24 2017

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Toll-Like Receptors
Luciferases
Mesenchymal Stromal Cells
Cytokines
Assays
Coculture Techniques
Genes
Experiments
Transcriptional Activation
Interleukin-6
Up-Regulation
Down-Regulation
Fluorescence
Cell Proliferation
Cell proliferation
Students
Cell Line
Messenger RNA
Extracellular Vesicles
Screening

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{a454517cb3324dc2924b9e3da2af1863,
title = "Erratum: Mesenchymal stromal cell-derived extracellular vesicles promote myeloid-biased multipotent hematopoietic progenitor expansion via Toll-like receptor engagement (The Journal of Biological Chemistry (2016) 291 (24607-24617) DOI: 10.1074/jbc.A116.745653)",
abstract = "There were several errors in the original figure legend to Fig. 4. The figure legend should be replaced with the following. FIGURE 4. MSC-derived EVs activate NF-κB and Notch signaling to promote HSPC differentiation. A, diagrammatic representation of the lentiviral vector design used to generate the NF-κB enhancer-luciferase reporter cell line. Stably transduced and sorted SIM-A9 microglial cells were used for EV exposure followed by luciferase assay. The SIM-A9-NF-κB-Luc cells were exposed with MSC EVs and incubated for 48 h followed by luciferase assay. Non-treated SIM-A9-NF-κB-Luc and LPS-treated SIM-A9-NF-κB-Luc cells were used as negative and positive controls, respectively. Luciferase activity was measured as relative fluorescence units (RFU). Data represent n = 4 independent experiments; error bars depict S.D. All p values have been calculated using two-tailed Student's t test. B, quantitative mRNA expression of NF-κB and Notch-1 signaling and downstream targets involved in cell proliferation in MSC EV-exposed HSPCs. Data represent n = 3 independent experiments; error bars are S.D. C, we next tested a candidate panel of canonical TLR4 responsive cytokines for both transcriptional activation and secretion, either after EV or S100 exposure. Transcriptional analysis of EV-exposed HSPCs reveals an up-regulation of several known TLR4-responsive cytokine genes (IL6, TNFa, Stat1, and EGFR) in WT but not MyD88-/- HSPCs. Data represent n = 3 independent experiments; error bars are S.D. D, TLR4 signaling was specifically inhibited in WT MSC-EV-exposed HSPCs using TAK-242. Transcriptional analysis of EV-exposed HSPCs with and without TLR4 inhibitor showed down-regulation of the same TLR-responsive genes described above. Data represent n = 2 independent experiments; error bars represent S.D. E, cytokines released by HSPCs after 48 h co-culture, with MSC EVs compared with S100, were measured using a Luminex assay, whereby error bars are S.D. and p values are an indication of the variance across three technical replicates in this screening panel.",
author = "Goloviznina, {Natalya A.} and Verghese, {Santhosh Chakkaramakkil} and Yoon, {Young Me} and Oleh Taratula and Daniel Marks and Peter Kurre",
year = "2017",
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language = "English (US)",
volume = "292",
pages = "3541",
journal = "Journal of Biological Chemistry",
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publisher = "American Society for Biochemistry and Molecular Biology Inc.",
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}

TY - JOUR

T1 - Erratum

T2 - Mesenchymal stromal cell-derived extracellular vesicles promote myeloid-biased multipotent hematopoietic progenitor expansion via Toll-like receptor engagement (The Journal of Biological Chemistry (2016) 291 (24607-24617) DOI: 10.1074/jbc.A116.745653)

AU - Goloviznina, Natalya A.

AU - Verghese, Santhosh Chakkaramakkil

AU - Yoon, Young Me

AU - Taratula, Oleh

AU - Marks, Daniel

AU - Kurre, Peter

PY - 2017/2/24

Y1 - 2017/2/24

N2 - There were several errors in the original figure legend to Fig. 4. The figure legend should be replaced with the following. FIGURE 4. MSC-derived EVs activate NF-κB and Notch signaling to promote HSPC differentiation. A, diagrammatic representation of the lentiviral vector design used to generate the NF-κB enhancer-luciferase reporter cell line. Stably transduced and sorted SIM-A9 microglial cells were used for EV exposure followed by luciferase assay. The SIM-A9-NF-κB-Luc cells were exposed with MSC EVs and incubated for 48 h followed by luciferase assay. Non-treated SIM-A9-NF-κB-Luc and LPS-treated SIM-A9-NF-κB-Luc cells were used as negative and positive controls, respectively. Luciferase activity was measured as relative fluorescence units (RFU). Data represent n = 4 independent experiments; error bars depict S.D. All p values have been calculated using two-tailed Student's t test. B, quantitative mRNA expression of NF-κB and Notch-1 signaling and downstream targets involved in cell proliferation in MSC EV-exposed HSPCs. Data represent n = 3 independent experiments; error bars are S.D. C, we next tested a candidate panel of canonical TLR4 responsive cytokines for both transcriptional activation and secretion, either after EV or S100 exposure. Transcriptional analysis of EV-exposed HSPCs reveals an up-regulation of several known TLR4-responsive cytokine genes (IL6, TNFa, Stat1, and EGFR) in WT but not MyD88-/- HSPCs. Data represent n = 3 independent experiments; error bars are S.D. D, TLR4 signaling was specifically inhibited in WT MSC-EV-exposed HSPCs using TAK-242. Transcriptional analysis of EV-exposed HSPCs with and without TLR4 inhibitor showed down-regulation of the same TLR-responsive genes described above. Data represent n = 2 independent experiments; error bars represent S.D. E, cytokines released by HSPCs after 48 h co-culture, with MSC EVs compared with S100, were measured using a Luminex assay, whereby error bars are S.D. and p values are an indication of the variance across three technical replicates in this screening panel.

AB - There were several errors in the original figure legend to Fig. 4. The figure legend should be replaced with the following. FIGURE 4. MSC-derived EVs activate NF-κB and Notch signaling to promote HSPC differentiation. A, diagrammatic representation of the lentiviral vector design used to generate the NF-κB enhancer-luciferase reporter cell line. Stably transduced and sorted SIM-A9 microglial cells were used for EV exposure followed by luciferase assay. The SIM-A9-NF-κB-Luc cells were exposed with MSC EVs and incubated for 48 h followed by luciferase assay. Non-treated SIM-A9-NF-κB-Luc and LPS-treated SIM-A9-NF-κB-Luc cells were used as negative and positive controls, respectively. Luciferase activity was measured as relative fluorescence units (RFU). Data represent n = 4 independent experiments; error bars depict S.D. All p values have been calculated using two-tailed Student's t test. B, quantitative mRNA expression of NF-κB and Notch-1 signaling and downstream targets involved in cell proliferation in MSC EV-exposed HSPCs. Data represent n = 3 independent experiments; error bars are S.D. C, we next tested a candidate panel of canonical TLR4 responsive cytokines for both transcriptional activation and secretion, either after EV or S100 exposure. Transcriptional analysis of EV-exposed HSPCs reveals an up-regulation of several known TLR4-responsive cytokine genes (IL6, TNFa, Stat1, and EGFR) in WT but not MyD88-/- HSPCs. Data represent n = 3 independent experiments; error bars are S.D. D, TLR4 signaling was specifically inhibited in WT MSC-EV-exposed HSPCs using TAK-242. Transcriptional analysis of EV-exposed HSPCs with and without TLR4 inhibitor showed down-regulation of the same TLR-responsive genes described above. Data represent n = 2 independent experiments; error bars represent S.D. E, cytokines released by HSPCs after 48 h co-culture, with MSC EVs compared with S100, were measured using a Luminex assay, whereby error bars are S.D. and p values are an indication of the variance across three technical replicates in this screening panel.

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U2 - 10.1074/jbc.M116.745653

DO - 10.1074/jbc.M116.745653

M3 - Comment/debate

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VL - 292

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 8

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