Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice

Goran Gregorovic; Elizabeth A. Boulden; Rachel Bosshard; Claudio Elgueta Karstegl; Rebecca Skalsky; Bryan R. Cullen; Cornelia Gujer; Patrick Rämer; Christian Münz; Paul J. Farrell

doi:10.1128/JVI.01873-15

Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice

Goran Gregorovic, Elizabeth A. Boulden, Rachel Bosshard, Claudio Elgueta Karstegl, Rebecca Skalsky, Bryan R. Cullen, Cornelia Gujer, Patrick Rämer, Christian Münz, Paul J. Farrell

Vaccine and Gene Therapy Institute

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.

Original language	English (US)
Pages (from-to)	11711-11714
Number of pages	4
Journal	Journal of virology
Volume	89
Issue number	22
DOIs	https://doi.org/10.1128/JVI.01873-15
State	Published - 2015

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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Gregorovic, G., Boulden, E. A., Bosshard, R., Karstegl, C. E., Skalsky, R., Cullen, B. R., Gujer, C., Rämer, P., Münz, C., & Farrell, P. J. (2015). Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice. Journal of virology, 89(22), 11711-11714. https://doi.org/10.1128/JVI.01873-15

Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice. / Gregorovic, Goran; Boulden, Elizabeth A.; Bosshard, Rachel et al.
In: Journal of virology, Vol. 89, No. 22, 2015, p. 11711-11714.

Research output: Contribution to journal › Article › peer-review

Gregorovic, G, Boulden, EA, Bosshard, R, Karstegl, CE, Skalsky, R, Cullen, BR, Gujer, C, Rämer, P, Münz, C & Farrell, PJ 2015, 'Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice', Journal of virology, vol. 89, no. 22, pp. 11711-11714. https://doi.org/10.1128/JVI.01873-15

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abstract = "Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.",

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AU - Gregorovic, Goran

AU - Boulden, Elizabeth A.

AU - Bosshard, Rachel

AU - Karstegl, Claudio Elgueta

AU - Skalsky, Rebecca

AU - Cullen, Bryan R.

AU - Gujer, Cornelia

AU - Rämer, Patrick

AU - Münz, Christian

AU - Farrell, Paul J.

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AB - Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.

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Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice

Abstract

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