Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice

Goran Gregorovic, Elizabeth A. Boulden, Rachel Bosshard, Claudio Elgueta Karstegl, Rebecca Skalsky, Bryan R. Cullen, Cornelia Gujer, Patrick Rämer, Christian Münz, Paul J. Farrell

Research output: Contribution to journalArticle

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Abstract

Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.

Original languageEnglish (US)
Pages (from-to)11711-11714
Number of pages4
JournalJournal of Virology
Volume89
Issue number22
DOIs
StatePublished - 2015

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Human herpesvirus 4
Human Herpesvirus 4
membrane proteins
Membrane Proteins
cell lines
RNA
Cell Line
mice
Infection
infection
ribosomal proteins
microRNA
Hematopoietic System
Ribosomal Proteins
MicroRNAs
mutants
proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

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Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice. / Gregorovic, Goran; Boulden, Elizabeth A.; Bosshard, Rachel; Karstegl, Claudio Elgueta; Skalsky, Rebecca; Cullen, Bryan R.; Gujer, Cornelia; Rämer, Patrick; Münz, Christian; Farrell, Paul J.

In: Journal of Virology, Vol. 89, No. 22, 2015, p. 11711-11714.

Research output: Contribution to journalArticle

Gregorovic, Goran ; Boulden, Elizabeth A. ; Bosshard, Rachel ; Karstegl, Claudio Elgueta ; Skalsky, Rebecca ; Cullen, Bryan R. ; Gujer, Cornelia ; Rämer, Patrick ; Münz, Christian ; Farrell, Paul J. / Epstein-Barr viruses (EBVs) deficient in EBV-encoded RNAs have higher levels of latent membrane protein 2 RNA expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice. In: Journal of Virology. 2015 ; Vol. 89, No. 22. pp. 11711-11714.
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AU - Boulden, Elizabeth A.

AU - Bosshard, Rachel

AU - Karstegl, Claudio Elgueta

AU - Skalsky, Rebecca

AU - Cullen, Bryan R.

AU - Gujer, Cornelia

AU - Rämer, Patrick

AU - Münz, Christian

AU - Farrell, Paul J.

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