Epoxidation of olf.fins by cytochromk P450 possible rolf, for hydroperoxo iron as the direct oxidant

A. D N Vaz, P. P. McGinnity, P. Zarehbin-Irani, M. J. Coon, J. P. Salaun, Dennis Koop, Bethany Klopfenstein

Research output: Contribution to journalArticle

Abstract

The epoxidalion of olefinic compounds by P450 cytochromes is a common reaction with importance in xenobiotic metabolism and an as yet unclear role in unsuturated tatty acid metabolism. This reaction is generally believed to involve tbe insertion of an oxygen atom from a hypervalcnt oxenoicl iron complex. To probe the mechanism of the reaction, we have examined the cpoxidation and hydroxylation of small olefins and unsaturated fatty acids by P450 2E1 and an active site Thr mutant (T303A) of 2E1 in a reconstituted system. The

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997
Externally publishedYes

Fingerprint

Epoxidation
Oxidants
Metabolism
oxidants
fins
Iron
iron
alkenes
Hydroxylation
metabolism
reaction mechanisms
Alkenes
hydroxylation
Xenobiotics
xenobiotics
Unsaturated Fatty Acids
cytochrome P-450
active sites
unsaturated fatty acids
Cytochrome P-450 Enzyme System

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Vaz, A. D. N., McGinnity, P. P., Zarehbin-Irani, P., Coon, M. J., Salaun, J. P., Koop, D., & Klopfenstein, B. (1997). Epoxidation of olf.fins by cytochromk P450 possible rolf, for hydroperoxo iron as the direct oxidant. FASEB Journal, 11(9).

Epoxidation of olf.fins by cytochromk P450 possible rolf, for hydroperoxo iron as the direct oxidant. / Vaz, A. D N; McGinnity, P. P.; Zarehbin-Irani, P.; Coon, M. J.; Salaun, J. P.; Koop, Dennis; Klopfenstein, Bethany.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Vaz ADN, McGinnity PP, Zarehbin-Irani P, Coon MJ, Salaun JP, Koop D et al. Epoxidation of olf.fins by cytochromk P450 possible rolf, for hydroperoxo iron as the direct oxidant. FASEB Journal. 1997;11(9).
Vaz, A. D N ; McGinnity, P. P. ; Zarehbin-Irani, P. ; Coon, M. J. ; Salaun, J. P. ; Koop, Dennis ; Klopfenstein, Bethany. / Epoxidation of olf.fins by cytochromk P450 possible rolf, for hydroperoxo iron as the direct oxidant. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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AU - Zarehbin-Irani, P.

AU - Coon, M. J.

AU - Salaun, J. P.

AU - Koop, Dennis

AU - Klopfenstein, Bethany

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