Epitopes of the human malaria parasite P. falciparum carried on the surface of HBsAg particles elicit an immune response against the parasite

Albrecht von Brunn, Klaus Frueh, Hans Michael Müller, Hans Walter Zentgraf, Hermann Bujard

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The development of recombinant subunit vaccines against pathogenic organisms requires not only the identification of epitopes eliciting a protective immune response but also suitable carriers with adjuvant function. B- and T-cell epitopes of the malaria vaccine candidate gp190 were selected on the basis of a systematic search along the gp190 molecule and by computer prediction based on the amino acid sequence. Using some of the epitopes identified, we have redesigned the surface of the hepatitis B surface antigen lipoprotein particles by replacing the major antigenic determinants with malaria-specific sequences of up to 61 amino acids in length. Upon expression via vaccinia virus the hybrid particles elicit an anti-gp190 immune response in animals. Monoclonal antibodies derived from such infections recognize the native parasite.

Original languageEnglish (US)
Pages (from-to)477-484
Number of pages8
JournalVaccine
Volume9
Issue number7
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Hepatitis B Surface Antigens
malaria
epitopes
Malaria
Epitopes
Parasites
immune response
parasites
Malaria Vaccines
B-Lymphocyte Epitopes
Synthetic Vaccines
Subunit Vaccines
T-Lymphocyte Epitopes
Vaccinia virus
Virion
Lipoproteins
subunit vaccines
Amino Acid Sequence
recombinant vaccines
Monoclonal Antibodies

Keywords

  • HBsAg-gp190 hybrids
  • Human malaria
  • immune response
  • merozoite surface antigen
  • selected epitopes

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Epitopes of the human malaria parasite P. falciparum carried on the surface of HBsAg particles elicit an immune response against the parasite. / von Brunn, Albrecht; Frueh, Klaus; Müller, Hans Michael; Zentgraf, Hans Walter; Bujard, Hermann.

In: Vaccine, Vol. 9, No. 7, 1991, p. 477-484.

Research output: Contribution to journalArticle

von Brunn, Albrecht ; Frueh, Klaus ; Müller, Hans Michael ; Zentgraf, Hans Walter ; Bujard, Hermann. / Epitopes of the human malaria parasite P. falciparum carried on the surface of HBsAg particles elicit an immune response against the parasite. In: Vaccine. 1991 ; Vol. 9, No. 7. pp. 477-484.
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