Epitope specificity of h-2kb-restricted, hsv-1-, and hsv-2-cross-reactive cytotoxic t lymphocyte clones

Robert H. Bonneau, Lisa A. Salvucci, David C. Johnson, Satvir S. Tevethia

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

HSV-1-specific and HSV-1/HSV-2-cross-reactive H-2Kb-restricted cytotoxic T lymphocyte (CTL) clones were derived from a pool of splenic memory CTL (CTLm) obtained from HSV-l-infected C57BL/6 mice. Two of the HSV-1/HSV-2-cross-reactive CTL clones recognized HSV gB since H-2b cells infected with a recombinant adenovirus vector expressing HSV glycoprotein B (gB) provided a target for these CTL clones. The CTL recognition epitope was precisely defined as HSV-1 gB residues 498-505 using synthetic peptides and conforms to a predicted H-2Kb-binding motif. Immunization of C57BL/6 mice with the free synthetic peptide corresponding to this predicted minimal epitope (HSV-1 gB498-505) resulted in the generation of HSV-gB epitope-specific CD8+ CTL in the popliteal lymph nodes. The peptide-induced CTL recognize and lyse HSV-1 infected H-2b cells or cells pulsed with the synthetic peptide, gB498-505. The availability of CTL clones directed to this predicted minimal HSV CTL epitope should be helpful in understanding processing of HSV glycoprotein B and presentation of this CTL recognition epitope.

Original languageEnglish (US)
Pages (from-to)62-70
Number of pages9
JournalVirology
Volume195
Issue number1
DOIs
StatePublished - Jul 1993

ASJC Scopus subject areas

  • Virology

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