Epithelial NF-κB enhances transmucosal fluid movement by altering tight junction protein composition after T cell activation

Yueming Tang, Daniel Clayburgh, Navdha Mittal, Tatiana Goretsky, Ramanarao Dirisina, Zheng Zhang, Michelle Kron, David Ivancic, Rebecca B. Katzman, Gery Grimm, Goo Lee, Jonathan Fryer, Asma Nusrat, Jerrold R. Turner, Terrence A. Barrett

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In inflammatory bowel disease (IBD), aberrant activation of innate and adaptive immune responses enhances mucosal permeability through mechanisms not completely understood. To examine the role of epithelial nuclear factor (NF-κB) in IBD-induced enhanced permeability, epithelial-specific IκBα mutant (NF-κB super repressor) transgenic (TG) mice were generated. NF-kB activation was inhibited in TG mice, relative to wild-type mice, following T cell-mediated immune cell activation using an anti-CD3 monoclonal antibody. Furthermore, epithelial NF-κB super repressor protein inhibited diarrhea and blocked changes in transepithelial resistance and transmucosal flux of alexa350 (0.35 kDa) and dextran3000 (3 kDa). In vivo perfusion loop studies in TG mice revealed reversed net water secretion and reduced lumenal flux of different molecular probes (bovine serum albumin, alexa350, and dextran3000). Cell-imaging and immunoblotting of low-density, detergent-insoluble membrane fractions confirmed that tight junction proteins (occludin, claudin-1 and zona occludens-1) are internalized through an NF-κB-dependent pathway. Taken together, these data suggest that IBD-associated diarrhea results from NF-κB-mediated tight junction protein internalization and increased paracellular permeability. Thus, reduction of epithelial NF-κB activation in IBD may repair defects in epithelial barrier function, reduce diarrhea, and limit protein (eg, serum albumin) losses. Epithelial NF-κB activation induced by mucosal T cells, therefore, actively plays a role in opening paracellular spaces to promote transmucosal fluid effux into the intestinal lumen.

Original languageEnglish (US)
Pages (from-to)158-167
Number of pages10
JournalAmerican Journal of Pathology
Volume176
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

Fingerprint

Tight Junction Proteins
Inflammatory Bowel Diseases
Transgenic Mice
T-Lymphocytes
Diarrhea
Permeability
Claudin-1
Repressor Proteins
Occludin
Molecular Probes
NF-kappa B
Herpes Zoster
Adaptive Immunity
Bovine Serum Albumin
Immunoblotting
Innate Immunity
Serum Albumin
Detergents
Perfusion
Monoclonal Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Epithelial NF-κB enhances transmucosal fluid movement by altering tight junction protein composition after T cell activation. / Tang, Yueming; Clayburgh, Daniel; Mittal, Navdha; Goretsky, Tatiana; Dirisina, Ramanarao; Zhang, Zheng; Kron, Michelle; Ivancic, David; Katzman, Rebecca B.; Grimm, Gery; Lee, Goo; Fryer, Jonathan; Nusrat, Asma; Turner, Jerrold R.; Barrett, Terrence A.

In: American Journal of Pathology, Vol. 176, No. 1, 01.2010, p. 158-167.

Research output: Contribution to journalArticle

Tang, Y, Clayburgh, D, Mittal, N, Goretsky, T, Dirisina, R, Zhang, Z, Kron, M, Ivancic, D, Katzman, RB, Grimm, G, Lee, G, Fryer, J, Nusrat, A, Turner, JR & Barrett, TA 2010, 'Epithelial NF-κB enhances transmucosal fluid movement by altering tight junction protein composition after T cell activation', American Journal of Pathology, vol. 176, no. 1, pp. 158-167. https://doi.org/10.2353/ajpath.2010.090548
Tang, Yueming ; Clayburgh, Daniel ; Mittal, Navdha ; Goretsky, Tatiana ; Dirisina, Ramanarao ; Zhang, Zheng ; Kron, Michelle ; Ivancic, David ; Katzman, Rebecca B. ; Grimm, Gery ; Lee, Goo ; Fryer, Jonathan ; Nusrat, Asma ; Turner, Jerrold R. ; Barrett, Terrence A. / Epithelial NF-κB enhances transmucosal fluid movement by altering tight junction protein composition after T cell activation. In: American Journal of Pathology. 2010 ; Vol. 176, No. 1. pp. 158-167.
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AU - Dirisina, Ramanarao

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AU - Kron, Michelle

AU - Ivancic, David

AU - Katzman, Rebecca B.

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AU - Lee, Goo

AU - Fryer, Jonathan

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N2 - In inflammatory bowel disease (IBD), aberrant activation of innate and adaptive immune responses enhances mucosal permeability through mechanisms not completely understood. To examine the role of epithelial nuclear factor (NF-κB) in IBD-induced enhanced permeability, epithelial-specific IκBα mutant (NF-κB super repressor) transgenic (TG) mice were generated. NF-kB activation was inhibited in TG mice, relative to wild-type mice, following T cell-mediated immune cell activation using an anti-CD3 monoclonal antibody. Furthermore, epithelial NF-κB super repressor protein inhibited diarrhea and blocked changes in transepithelial resistance and transmucosal flux of alexa350 (0.35 kDa) and dextran3000 (3 kDa). In vivo perfusion loop studies in TG mice revealed reversed net water secretion and reduced lumenal flux of different molecular probes (bovine serum albumin, alexa350, and dextran3000). Cell-imaging and immunoblotting of low-density, detergent-insoluble membrane fractions confirmed that tight junction proteins (occludin, claudin-1 and zona occludens-1) are internalized through an NF-κB-dependent pathway. Taken together, these data suggest that IBD-associated diarrhea results from NF-κB-mediated tight junction protein internalization and increased paracellular permeability. Thus, reduction of epithelial NF-κB activation in IBD may repair defects in epithelial barrier function, reduce diarrhea, and limit protein (eg, serum albumin) losses. Epithelial NF-κB activation induced by mucosal T cells, therefore, actively plays a role in opening paracellular spaces to promote transmucosal fluid effux into the intestinal lumen.

AB - In inflammatory bowel disease (IBD), aberrant activation of innate and adaptive immune responses enhances mucosal permeability through mechanisms not completely understood. To examine the role of epithelial nuclear factor (NF-κB) in IBD-induced enhanced permeability, epithelial-specific IκBα mutant (NF-κB super repressor) transgenic (TG) mice were generated. NF-kB activation was inhibited in TG mice, relative to wild-type mice, following T cell-mediated immune cell activation using an anti-CD3 monoclonal antibody. Furthermore, epithelial NF-κB super repressor protein inhibited diarrhea and blocked changes in transepithelial resistance and transmucosal flux of alexa350 (0.35 kDa) and dextran3000 (3 kDa). In vivo perfusion loop studies in TG mice revealed reversed net water secretion and reduced lumenal flux of different molecular probes (bovine serum albumin, alexa350, and dextran3000). Cell-imaging and immunoblotting of low-density, detergent-insoluble membrane fractions confirmed that tight junction proteins (occludin, claudin-1 and zona occludens-1) are internalized through an NF-κB-dependent pathway. Taken together, these data suggest that IBD-associated diarrhea results from NF-κB-mediated tight junction protein internalization and increased paracellular permeability. Thus, reduction of epithelial NF-κB activation in IBD may repair defects in epithelial barrier function, reduce diarrhea, and limit protein (eg, serum albumin) losses. Epithelial NF-κB activation induced by mucosal T cells, therefore, actively plays a role in opening paracellular spaces to promote transmucosal fluid effux into the intestinal lumen.

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