Epinephrine regulation of skeletal muscle glycogen metabolism. Studies utilizing the perfused rat hindlimb preparation

M. R. Dietz, J. L. Chiasson, Thomas Soderling, J. H. Exton

Research output: Contribution to journalArticle

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Abstract

Studies of rat skeletal muscle glycogen metabolism carried out in a perfused hindlimb system indicated that epinephrine activates phosphorylase via the cascade of phosphorylation reactions classically linked to the β-adrenergic receptor/adenylate cyclase system. The β blocker propranolol completely blocked the effects of epinephrine on cAMP, cAMP-dependent protein kinase, phosphorylase, and glucose-6-P, whereas the α blocker phentolamine was totally ineffective. Omission of glucose from the perfusion medium did not modify the effects of epinephrine. Glycogen synthase activity in control perfused and nonperfused muscle was largely glucose-6-P-dependent (-glucose-6-P/+glucose-6-P activity ratios of 0.1 and 0.2, respectively). Epinephrine perfusion caused a small decrease in the enzyme's activity ratio (0.1 to 0.05) and a large increase in its K(a) for glucose-6-P (0.3 to 1.5 mM). This increase in glucose-6-P dependency correlated in time with protein kinase activation and was totally blocked by propranolol and unaffected by phentolamine. Comparison of the kinetics of glycogen synthase in extracts of control and epinephrine-perfused muscle with the kinetics of purified rat skeletal muscle glycogen synthase a phosphorylated to various degrees by cAMP-dependent protein kinase indicated that the enzyme was already substantially phosphorylated in control muscle and that epinephrine treatment caused further phosphorylation of synthase, presumably via camp-dependent protein kinase. These data provide a basis for speculation about in vivo regulation of the enzyme.

Original languageEnglish (US)
Pages (from-to)2301-2307
Number of pages7
JournalJournal of Biological Chemistry
Volume255
Issue number6
StatePublished - 1980
Externally publishedYes

Fingerprint

Hindlimb
Glycogen
Metabolism
Epinephrine
Muscle
Rats
Skeletal Muscle
Glucose
Glycogen Synthase
Phosphorylases
Phosphorylation
Phentolamine
Cyclic AMP-Dependent Protein Kinases
Propranolol
Muscles
Protein Kinases
Enzymes
Perfusion
Kinetics
Enzyme activity

ASJC Scopus subject areas

  • Biochemistry

Cite this

Epinephrine regulation of skeletal muscle glycogen metabolism. Studies utilizing the perfused rat hindlimb preparation. / Dietz, M. R.; Chiasson, J. L.; Soderling, Thomas; Exton, J. H.

In: Journal of Biological Chemistry, Vol. 255, No. 6, 1980, p. 2301-2307.

Research output: Contribution to journalArticle

Dietz, M. R. ; Chiasson, J. L. ; Soderling, Thomas ; Exton, J. H. / Epinephrine regulation of skeletal muscle glycogen metabolism. Studies utilizing the perfused rat hindlimb preparation. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 6. pp. 2301-2307.
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