Environmental stressors influence limited-access ethanol consumption by C57BL/6J mice in a sex-dependent manner

Debra K. Cozzoli, Michelle A. Tanchuck-Nipper, Moriah N. Kaufman, Chloe B. Horowitz, Deborah (Deb) Finn

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Exposure to stress contributes to ethanol consumption in humans, but it produces inconsistent effects on ethanol drinking in rodent models. Therefore, the present study examined the influence of different stressors (restraint, tail suspension, predator odor, foot shock, and tail pinch) on 2-h access to water and 10% ethanol by male and female C57BL/6J mice and determined whether there were sex-dependent differences in response to stress. Plasma corticosterone (CORT) and allopregnanolone (ALLO) were assessed as indexes of hypothalamic-pituitary-adrenal axis activity and of endogenous neurosteroid levels, respectively, following restraint, tail suspension, and predator odor. These stressors increased plasma CORT and ALLO levels, and produced a greater increase in CORT and ALLO levels in females versus males. Ethanol intake was decreased following restraint, tail suspension, foot shock, and tail pinch in both sexes, with stressor-related differences in the duration of the suppression. Predator odor significantly increased ethanol intake on the following two days in females and on the second day after stress in males. Notably, there was a significant positive correlation between CORT levels immediately after predator odor stress and ethanol intake on the following day. In summary, the type of stressor influenced ethanol consumption, with subtle sex differences in the magnitude and persistence of the effect. These findings are the first to demonstrate that a single, acute exposure to restraint, tail suspension, and predator odor stress increased plasma CORT and ALLO levels in animals with a history of ethanol consumption and that female mice were more responsive than males to the ability of stress to increase CORT and ALLO levels as well as to increase ethanol intake following predator odor stress. Because predator odor stress is a model of post-traumatic stress disorder (PTSD), the present sex differences have important implications for preclinical studies modeling the comorbidity of PTSD and alcohol use disorders.

Original languageEnglish (US)
Pages (from-to)741-754
Number of pages14
JournalAlcohol
Volume48
Issue number8
DOIs
StatePublished - Dec 1 2014

Fingerprint

Inbred C57BL Mouse
Odors
Ethanol
Pregnanolone
Corticosterone
Hindlimb Suspension
Suspensions
Sex Characteristics
posttraumatic stress disorder
Post-Traumatic Stress Disorders
Plasmas
Tail
Foot
Shock
comorbidity
Aptitude
suppression
Odorants
persistence
Drinking

Keywords

  • Allopregnanolone
  • Corticosterone
  • Environmental stress
  • Foot shock
  • Predator odor
  • Tail pinch

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neurology
  • Toxicology
  • Health(social science)

Cite this

Environmental stressors influence limited-access ethanol consumption by C57BL/6J mice in a sex-dependent manner. / Cozzoli, Debra K.; Tanchuck-Nipper, Michelle A.; Kaufman, Moriah N.; Horowitz, Chloe B.; Finn, Deborah (Deb).

In: Alcohol, Vol. 48, No. 8, 01.12.2014, p. 741-754.

Research output: Contribution to journalArticle

Cozzoli, Debra K. ; Tanchuck-Nipper, Michelle A. ; Kaufman, Moriah N. ; Horowitz, Chloe B. ; Finn, Deborah (Deb). / Environmental stressors influence limited-access ethanol consumption by C57BL/6J mice in a sex-dependent manner. In: Alcohol. 2014 ; Vol. 48, No. 8. pp. 741-754.
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AU - Horowitz, Chloe B.

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AB - Exposure to stress contributes to ethanol consumption in humans, but it produces inconsistent effects on ethanol drinking in rodent models. Therefore, the present study examined the influence of different stressors (restraint, tail suspension, predator odor, foot shock, and tail pinch) on 2-h access to water and 10% ethanol by male and female C57BL/6J mice and determined whether there were sex-dependent differences in response to stress. Plasma corticosterone (CORT) and allopregnanolone (ALLO) were assessed as indexes of hypothalamic-pituitary-adrenal axis activity and of endogenous neurosteroid levels, respectively, following restraint, tail suspension, and predator odor. These stressors increased plasma CORT and ALLO levels, and produced a greater increase in CORT and ALLO levels in females versus males. Ethanol intake was decreased following restraint, tail suspension, foot shock, and tail pinch in both sexes, with stressor-related differences in the duration of the suppression. Predator odor significantly increased ethanol intake on the following two days in females and on the second day after stress in males. Notably, there was a significant positive correlation between CORT levels immediately after predator odor stress and ethanol intake on the following day. In summary, the type of stressor influenced ethanol consumption, with subtle sex differences in the magnitude and persistence of the effect. These findings are the first to demonstrate that a single, acute exposure to restraint, tail suspension, and predator odor stress increased plasma CORT and ALLO levels in animals with a history of ethanol consumption and that female mice were more responsive than males to the ability of stress to increase CORT and ALLO levels as well as to increase ethanol intake following predator odor stress. Because predator odor stress is a model of post-traumatic stress disorder (PTSD), the present sex differences have important implications for preclinical studies modeling the comorbidity of PTSD and alcohol use disorders.

KW - Allopregnanolone

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