Environmental amoebae and mycobacterial pathogenesis

Melanie Harriff, Luiz E. Bermudez

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Environmental amoebae have been shown to be a host to pathogenic mycobacteria. Mycobacterium avium, Mycobacterium marinum, and Mycobacterium peregrinum can all grow inside Acanthamoeba and other environmental amoebae. Once ingested by Acanthamoeba, M. avium upregulates a number of genes, many of them similar to genes upregulated upon phagocytosis of M. avium by macrophages. Mycobacteria ingested by amoebae grow intracellularly, acquiring an invasive phenotype, evident when the bacterium escapes the infected amoeba. Once inside of amoeba, it has been shown that mycobacteria are protected from antibiotics and disinfectants, such as chlorine. This chapter describes methods employed for the study of the interaction of M. avium and Acanthamoeba.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages433-442
Number of pages10
Volume465
DOIs
StatePublished - 2008

Publication series

NameMethods in Molecular Biology
Volume465
ISSN (Print)10643745

Fingerprint

Amoeba
Mycobacterium avium
Mycobacterium
Acanthamoeba
Mycobacterium marinum
Disinfectants
Chlorine
Phagocytosis
Genes
Up-Regulation
Macrophages
Anti-Bacterial Agents
Bacteria
Phenotype

Keywords

  • Acanthamoeba
  • Increase of virulence
  • Intracellular
  • Macrophage
  • Mucosal epithelial cell
  • Mycobacterium avium
  • Mycobacterium marinum
  • Mycobacterium peregrinum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Harriff, M., & Bermudez, L. E. (2008). Environmental amoebae and mycobacterial pathogenesis. In Methods in Molecular Biology (Vol. 465, pp. 433-442). (Methods in Molecular Biology; Vol. 465). https://doi.org/10.1007/978-1-59745-207-6_28

Environmental amoebae and mycobacterial pathogenesis. / Harriff, Melanie; Bermudez, Luiz E.

Methods in Molecular Biology. Vol. 465 2008. p. 433-442 (Methods in Molecular Biology; Vol. 465).

Research output: Chapter in Book/Report/Conference proceedingChapter

Harriff, M & Bermudez, LE 2008, Environmental amoebae and mycobacterial pathogenesis. in Methods in Molecular Biology. vol. 465, Methods in Molecular Biology, vol. 465, pp. 433-442. https://doi.org/10.1007/978-1-59745-207-6_28
Harriff M, Bermudez LE. Environmental amoebae and mycobacterial pathogenesis. In Methods in Molecular Biology. Vol. 465. 2008. p. 433-442. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-207-6_28
Harriff, Melanie ; Bermudez, Luiz E. / Environmental amoebae and mycobacterial pathogenesis. Methods in Molecular Biology. Vol. 465 2008. pp. 433-442 (Methods in Molecular Biology).
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