Enhancement of primate oocyte maturation and fertilization in vitro by inhibin A and activin A

B. M. Alak, G. D. Smith, T. K. Woodruff, Richard Stouffer, D. P. Wolf

    Research output: Contribution to journalArticle

    75 Citations (Scopus)

    Abstract

    Objective: A role for inhibin and activin in primate oocyte maturation was investigated. Design: The maturation and fertilization of rhesus monkey oocytes recovered from the excised ovaries of nine regularly cycling animals was compared for untreated germinal vesicle (GV)intact controls versus oocytes cultured in the presence of inhibin, activin, inhibin + activin, or in a combination with follistatin. Setting: Nonhuman primates in a research institute environment. Interventions: Bilateral oophorectomy. Main Outcome Measure: Oocyte maturation from germinal vesicle breakdown (GVBD) to metaphase II (MII) and fertilization. Results: Germinal vesicle breakdown, progression to MII and fertilization was monitored in oocytes cultured for 48 hours. Activin alone, at an optimum concentration of 100 ng/mL, stimulated GVBD whereas both GVBD and MII development was enhanced in the presence of inhibin activin. The latter also accelerated the rate of maturation to MII. All treatment groups exhibited a higher incidence of GVBD compared with controls. When follistatin was added, the stimulatory effect of activin or activin + inhibin was abolished. Exposure to medium containing inhibin + activin significantly increased the percentage of MII oocytes that fertilized compared with controls (68% versus 25%, respectively). Conclusions: Inhibin and activin are potent stimulators of primate oocyte maturation, producing mature oocytes in vitro that fertilize with high efficiency.

    Original languageEnglish (US)
    Pages (from-to)646-653
    Number of pages8
    JournalFertility and Sterility
    Volume66
    Issue number4
    StatePublished - 1996

    Fingerprint

    Activins
    Fertilization in Vitro
    Inhibins
    Primates
    Oocytes
    Metaphase
    Fertilization
    Follistatin
    activin A
    inhibin A
    Ovariectomy
    Macaca mulatta
    Ovary
    Outcome Assessment (Health Care)

    Keywords

    • activin
    • in vitro fertilization
    • Inhibin
    • Macaca mulatta
    • oocyte maturation

    ASJC Scopus subject areas

    • Obstetrics and Gynecology

    Cite this

    Enhancement of primate oocyte maturation and fertilization in vitro by inhibin A and activin A. / Alak, B. M.; Smith, G. D.; Woodruff, T. K.; Stouffer, Richard; Wolf, D. P.

    In: Fertility and Sterility, Vol. 66, No. 4, 1996, p. 646-653.

    Research output: Contribution to journalArticle

    Alak, B. M. ; Smith, G. D. ; Woodruff, T. K. ; Stouffer, Richard ; Wolf, D. P. / Enhancement of primate oocyte maturation and fertilization in vitro by inhibin A and activin A. In: Fertility and Sterility. 1996 ; Vol. 66, No. 4. pp. 646-653.
    @article{bfdee733ed9d41f8b0f3de473c4cf8c3,
    title = "Enhancement of primate oocyte maturation and fertilization in vitro by inhibin A and activin A",
    abstract = "Objective: A role for inhibin and activin in primate oocyte maturation was investigated. Design: The maturation and fertilization of rhesus monkey oocytes recovered from the excised ovaries of nine regularly cycling animals was compared for untreated germinal vesicle (GV)intact controls versus oocytes cultured in the presence of inhibin, activin, inhibin + activin, or in a combination with follistatin. Setting: Nonhuman primates in a research institute environment. Interventions: Bilateral oophorectomy. Main Outcome Measure: Oocyte maturation from germinal vesicle breakdown (GVBD) to metaphase II (MII) and fertilization. Results: Germinal vesicle breakdown, progression to MII and fertilization was monitored in oocytes cultured for 48 hours. Activin alone, at an optimum concentration of 100 ng/mL, stimulated GVBD whereas both GVBD and MII development was enhanced in the presence of inhibin activin. The latter also accelerated the rate of maturation to MII. All treatment groups exhibited a higher incidence of GVBD compared with controls. When follistatin was added, the stimulatory effect of activin or activin + inhibin was abolished. Exposure to medium containing inhibin + activin significantly increased the percentage of MII oocytes that fertilized compared with controls (68{\%} versus 25{\%}, respectively). Conclusions: Inhibin and activin are potent stimulators of primate oocyte maturation, producing mature oocytes in vitro that fertilize with high efficiency.",
    keywords = "activin, in vitro fertilization, Inhibin, Macaca mulatta, oocyte maturation",
    author = "Alak, {B. M.} and Smith, {G. D.} and Woodruff, {T. K.} and Richard Stouffer and Wolf, {D. P.}",
    year = "1996",
    language = "English (US)",
    volume = "66",
    pages = "646--653",
    journal = "Fertility and Sterility",
    issn = "0015-0282",
    publisher = "Elsevier Inc.",
    number = "4",

    }

    TY - JOUR

    T1 - Enhancement of primate oocyte maturation and fertilization in vitro by inhibin A and activin A

    AU - Alak, B. M.

    AU - Smith, G. D.

    AU - Woodruff, T. K.

    AU - Stouffer, Richard

    AU - Wolf, D. P.

    PY - 1996

    Y1 - 1996

    N2 - Objective: A role for inhibin and activin in primate oocyte maturation was investigated. Design: The maturation and fertilization of rhesus monkey oocytes recovered from the excised ovaries of nine regularly cycling animals was compared for untreated germinal vesicle (GV)intact controls versus oocytes cultured in the presence of inhibin, activin, inhibin + activin, or in a combination with follistatin. Setting: Nonhuman primates in a research institute environment. Interventions: Bilateral oophorectomy. Main Outcome Measure: Oocyte maturation from germinal vesicle breakdown (GVBD) to metaphase II (MII) and fertilization. Results: Germinal vesicle breakdown, progression to MII and fertilization was monitored in oocytes cultured for 48 hours. Activin alone, at an optimum concentration of 100 ng/mL, stimulated GVBD whereas both GVBD and MII development was enhanced in the presence of inhibin activin. The latter also accelerated the rate of maturation to MII. All treatment groups exhibited a higher incidence of GVBD compared with controls. When follistatin was added, the stimulatory effect of activin or activin + inhibin was abolished. Exposure to medium containing inhibin + activin significantly increased the percentage of MII oocytes that fertilized compared with controls (68% versus 25%, respectively). Conclusions: Inhibin and activin are potent stimulators of primate oocyte maturation, producing mature oocytes in vitro that fertilize with high efficiency.

    AB - Objective: A role for inhibin and activin in primate oocyte maturation was investigated. Design: The maturation and fertilization of rhesus monkey oocytes recovered from the excised ovaries of nine regularly cycling animals was compared for untreated germinal vesicle (GV)intact controls versus oocytes cultured in the presence of inhibin, activin, inhibin + activin, or in a combination with follistatin. Setting: Nonhuman primates in a research institute environment. Interventions: Bilateral oophorectomy. Main Outcome Measure: Oocyte maturation from germinal vesicle breakdown (GVBD) to metaphase II (MII) and fertilization. Results: Germinal vesicle breakdown, progression to MII and fertilization was monitored in oocytes cultured for 48 hours. Activin alone, at an optimum concentration of 100 ng/mL, stimulated GVBD whereas both GVBD and MII development was enhanced in the presence of inhibin activin. The latter also accelerated the rate of maturation to MII. All treatment groups exhibited a higher incidence of GVBD compared with controls. When follistatin was added, the stimulatory effect of activin or activin + inhibin was abolished. Exposure to medium containing inhibin + activin significantly increased the percentage of MII oocytes that fertilized compared with controls (68% versus 25%, respectively). Conclusions: Inhibin and activin are potent stimulators of primate oocyte maturation, producing mature oocytes in vitro that fertilize with high efficiency.

    KW - activin

    KW - in vitro fertilization

    KW - Inhibin

    KW - Macaca mulatta

    KW - oocyte maturation

    UR - http://www.scopus.com/inward/record.url?scp=0029790576&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0029790576&partnerID=8YFLogxK

    M3 - Article

    VL - 66

    SP - 646

    EP - 653

    JO - Fertility and Sterility

    JF - Fertility and Sterility

    SN - 0015-0282

    IS - 4

    ER -