Enhanced visibility of colonic neoplasms using formulaic ratio imaging of native fluorescence

Bhaskar Banerjee, Nathaniel S. Rial, Timothy Renkoski, Logan R. Graves, Sirandon A H Reid, Chengcheng Hu, Vassiliki Tsikitis, Valentine Nfonsom, Judith Pugh, Urs Utzinger

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background and Objectives Colonoscopy is the preferred method for colon cancer screening, but can miss polyps and flat neoplasms with low color contrast. The objective was to develop a new autofluorescence method that improves image contrast of colonic neoplasms. Study Design/Materials and Methods We selected the three strongest native fluorescence signals and developed a novel method where fluorescence images are processed in a ratiometric formula to represent the likely cellular and structural changes associated with neoplasia. Native fluorescence images of fresh surgical specimens of the colon containing normal mucosa, polypoid and flat adenomas as well as adenocarcinoma were recorded using a prototype multi-spectral imager. Sixteen patients, with a mean age of 62 years (range 28-81) undergoing elective resection for colonic neoplasms were enrolled. High contrast images were seen with fluorescence from tryptophan (Tryp), flavin adenine dinucleotide (FAD) and collagen. Results When the image intensity of Tryp was divided pixel by pixel, by the intensities of FAD and collagen, the resulting formulaic ratio (FR) images were of exceptionally high contrast. The FR images of adenomas and adenocarcinomas had increased Weber contrast. Conclusions FR imaging is a novel imaging process that represents the likely metabolic and structural changes in colonic neoplasia that produces images with remarkably high contrast. Lasers Surg. Med. 45:573-581, 2013.

Original languageEnglish (US)
Pages (from-to)573-581
Number of pages9
JournalLasers in Surgery and Medicine
Volume45
Issue number9
DOIs
StatePublished - Nov 2013
Externally publishedYes

Fingerprint

Optical Imaging
Colonic Neoplasms
Fluorescence
Flavin-Adenine Dinucleotide
Tryptophan
Adenoma
Adenocarcinoma
Collagen
Neoplasms
Colonoscopy
Polyps
Early Detection of Cancer
Colon
Mucous Membrane
Lasers
Color

Keywords

  • cancer
  • colon
  • fluorescence
  • imaging
  • polyps

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Banerjee, B., Rial, N. S., Renkoski, T., Graves, L. R., Reid, S. A. H., Hu, C., ... Utzinger, U. (2013). Enhanced visibility of colonic neoplasms using formulaic ratio imaging of native fluorescence. Lasers in Surgery and Medicine, 45(9), 573-581. https://doi.org/10.1002/lsm.22186

Enhanced visibility of colonic neoplasms using formulaic ratio imaging of native fluorescence. / Banerjee, Bhaskar; Rial, Nathaniel S.; Renkoski, Timothy; Graves, Logan R.; Reid, Sirandon A H; Hu, Chengcheng; Tsikitis, Vassiliki; Nfonsom, Valentine; Pugh, Judith; Utzinger, Urs.

In: Lasers in Surgery and Medicine, Vol. 45, No. 9, 11.2013, p. 573-581.

Research output: Contribution to journalArticle

Banerjee, B, Rial, NS, Renkoski, T, Graves, LR, Reid, SAH, Hu, C, Tsikitis, V, Nfonsom, V, Pugh, J & Utzinger, U 2013, 'Enhanced visibility of colonic neoplasms using formulaic ratio imaging of native fluorescence', Lasers in Surgery and Medicine, vol. 45, no. 9, pp. 573-581. https://doi.org/10.1002/lsm.22186
Banerjee, Bhaskar ; Rial, Nathaniel S. ; Renkoski, Timothy ; Graves, Logan R. ; Reid, Sirandon A H ; Hu, Chengcheng ; Tsikitis, Vassiliki ; Nfonsom, Valentine ; Pugh, Judith ; Utzinger, Urs. / Enhanced visibility of colonic neoplasms using formulaic ratio imaging of native fluorescence. In: Lasers in Surgery and Medicine. 2013 ; Vol. 45, No. 9. pp. 573-581.
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AB - Background and Objectives Colonoscopy is the preferred method for colon cancer screening, but can miss polyps and flat neoplasms with low color contrast. The objective was to develop a new autofluorescence method that improves image contrast of colonic neoplasms. Study Design/Materials and Methods We selected the three strongest native fluorescence signals and developed a novel method where fluorescence images are processed in a ratiometric formula to represent the likely cellular and structural changes associated with neoplasia. Native fluorescence images of fresh surgical specimens of the colon containing normal mucosa, polypoid and flat adenomas as well as adenocarcinoma were recorded using a prototype multi-spectral imager. Sixteen patients, with a mean age of 62 years (range 28-81) undergoing elective resection for colonic neoplasms were enrolled. High contrast images were seen with fluorescence from tryptophan (Tryp), flavin adenine dinucleotide (FAD) and collagen. Results When the image intensity of Tryp was divided pixel by pixel, by the intensities of FAD and collagen, the resulting formulaic ratio (FR) images were of exceptionally high contrast. The FR images of adenomas and adenocarcinomas had increased Weber contrast. Conclusions FR imaging is a novel imaging process that represents the likely metabolic and structural changes in colonic neoplasia that produces images with remarkably high contrast. Lasers Surg. Med. 45:573-581, 2013.

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