Enhanced T-Helper Cell Function Following CD4 Modulation

William J. Morrison, Halina Offner, Arthur Vandenbark

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

T cells made CD4- by ganglioside (GMI) treatment were cultured in antisense oligodeoxynucleotides complementary to mRNA for CD4. Antisense treatments decreased CD4 levels by 45% but did not prolong the total CD4 modulation by GMI pretreatment. Northern blot analysis demonstrated that CD4 message production did not change after ganglioside modulation and consequently that it was different from antisense CD4 modulation. However, modulation of CD4 from the cell surface by either GM1 or antisense resulted in greater proliferation and enhanced DTH when challenged with recall antigen. These results demonstrate that selective decrease of CD4 increased antigen-specific T cell responses.

Original languageEnglish (US)
Pages (from-to)392-400
Number of pages9
JournalCellular Immunology
Volume153
Issue number2
DOIs
StatePublished - Feb 1994

Fingerprint

Gangliosides
Helper-Inducer T-Lymphocytes
T-Lymphocytes
CD4 Antigens
Oligodeoxyribonucleotides
Northern Blotting
Antigens
Messenger RNA

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Enhanced T-Helper Cell Function Following CD4 Modulation. / Morrison, William J.; Offner, Halina; Vandenbark, Arthur.

In: Cellular Immunology, Vol. 153, No. 2, 02.1994, p. 392-400.

Research output: Contribution to journalArticle

@article{f65a60508cb742cd83c8455de2addb64,
title = "Enhanced T-Helper Cell Function Following CD4 Modulation",
abstract = "T cells made CD4- by ganglioside (GMI) treatment were cultured in antisense oligodeoxynucleotides complementary to mRNA for CD4. Antisense treatments decreased CD4 levels by 45{\%} but did not prolong the total CD4 modulation by GMI pretreatment. Northern blot analysis demonstrated that CD4 message production did not change after ganglioside modulation and consequently that it was different from antisense CD4 modulation. However, modulation of CD4 from the cell surface by either GM1 or antisense resulted in greater proliferation and enhanced DTH when challenged with recall antigen. These results demonstrate that selective decrease of CD4 increased antigen-specific T cell responses.",
author = "Morrison, {William J.} and Halina Offner and Arthur Vandenbark",
year = "1994",
month = "2",
doi = "10.1006/cimm.1994.1037",
language = "English (US)",
volume = "153",
pages = "392--400",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Enhanced T-Helper Cell Function Following CD4 Modulation

AU - Morrison, William J.

AU - Offner, Halina

AU - Vandenbark, Arthur

PY - 1994/2

Y1 - 1994/2

N2 - T cells made CD4- by ganglioside (GMI) treatment were cultured in antisense oligodeoxynucleotides complementary to mRNA for CD4. Antisense treatments decreased CD4 levels by 45% but did not prolong the total CD4 modulation by GMI pretreatment. Northern blot analysis demonstrated that CD4 message production did not change after ganglioside modulation and consequently that it was different from antisense CD4 modulation. However, modulation of CD4 from the cell surface by either GM1 or antisense resulted in greater proliferation and enhanced DTH when challenged with recall antigen. These results demonstrate that selective decrease of CD4 increased antigen-specific T cell responses.

AB - T cells made CD4- by ganglioside (GMI) treatment were cultured in antisense oligodeoxynucleotides complementary to mRNA for CD4. Antisense treatments decreased CD4 levels by 45% but did not prolong the total CD4 modulation by GMI pretreatment. Northern blot analysis demonstrated that CD4 message production did not change after ganglioside modulation and consequently that it was different from antisense CD4 modulation. However, modulation of CD4 from the cell surface by either GM1 or antisense resulted in greater proliferation and enhanced DTH when challenged with recall antigen. These results demonstrate that selective decrease of CD4 increased antigen-specific T cell responses.

UR - http://www.scopus.com/inward/record.url?scp=0028217215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028217215&partnerID=8YFLogxK

U2 - 10.1006/cimm.1994.1037

DO - 10.1006/cimm.1994.1037

M3 - Article

C2 - 8118871

AN - SCOPUS:0028217215

VL - 153

SP - 392

EP - 400

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 2

ER -