Enhanced sensitivity of Neil1−/− mice to chronic UVB exposure

Marcus J. Calkins, Vladimir Vartanian, Nichole Owen, Guldal Kirkali, Pawel Jaruga, Miral Dizdaroglu, Amanda McCullough, Robert (Stephen) Lloyd

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Oxidative stress and reactive oxygen species (ROS)-induced DNA base damage are thought to be central mediators of UV-induced carcinogenesis and skin aging. However, increased steady-state levels of ROS-induced DNA base damage have not been reported after chronic UV exposure. Accumulation of ROS-induced DNA base damage is governed by rates of lesion formation and repair. Repair is generally performed by Base Excision Repair (BER), which is initiated by DNA glycosylases, such as 8-oxoguanine glycosylase and Nei-Endonuclease VIII-Like 1 (NEIL1). In the current study, UV light (UVB) was used to elicit protracted low-level ROS challenge in wild-type (WT) and Neil1−/− mouse skin. Relative to WT controls, Neil1−/− mice showed an increased sensitivity to tissue destruction from the chronic UVB exposure, and corresponding enhanced chronic inflammatory responses as measured by cytokine message levels and profiling, as well as neutrophil infiltration. Additionally, levels of several ROS-induced DNA lesions were measured including 4,6-diamino-5-formamidopyrimidine (FapyGua), 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyAde), 8-hydroxyguanine (8-OH-Gua), 5,6-dihydroxyuracil (5,6-diOH-Ura) and thymine glycol (ThyGly). In WT mice, chronic UVB exposure led to increased steady-state levels of FapyGua, FapyAde, and ThyGly with no significant increases in 8-OH-Gua or 5,6-diOH-Ura. Interestingly, the lesions that accumulated were all substrates of NEIL1. Collectively, these data suggest that NEIL1-initiated repair of a subset of ROS-induced DNA base lesions may be insufficient to prevent the initiation of inflammatory pathways during chronic UV exposure in mouse skin.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalDNA Repair
Volume48
DOIs
StatePublished - Dec 1 2016

Keywords

  • DNA base excision repair
  • DNA glycosylases
  • Oxidatively-induced DNA base damage
  • UV-induced inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Calkins, M. J., Vartanian, V., Owen, N., Kirkali, G., Jaruga, P., Dizdaroglu, M., McCullough, A., & Lloyd, R. S. (2016). Enhanced sensitivity of Neil1−/− mice to chronic UVB exposure. DNA Repair, 48, 43-50. https://doi.org/10.1016/j.dnarep.2016.10.010