Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions

Taeseok Oh; Jae Young Choi; Michael J. Heller

doi:10.1039/c5an02516h

Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions

Taeseok Oh, Jae Young Choi, Michael J. Heller

Knight Cancer Institute

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Dimerization and resultant quenching of donor and acceptor dyes conjugated on DNA causes loss of fluorescent resonant energy transfer (FRET) efficiency. However, when complexed with surfactants and divalent metal ions, sheathing effects insulate and shield the DNA structures, reducing dimerization and quenching which leads to significant enhancement of FRET efficiency.

Original language	English (US)
Pages (from-to)	2371-2375
Number of pages	5
Journal	Analyst
Volume	141
Issue number	8
DOIs	https://doi.org/10.1039/c5an02516h
State	Published - Apr 21 2016

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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abstract = "Dimerization and resultant quenching of donor and acceptor dyes conjugated on DNA causes loss of fluorescent resonant energy transfer (FRET) efficiency. However, when complexed with surfactants and divalent metal ions, sheathing effects insulate and shield the DNA structures, reducing dimerization and quenching which leads to significant enhancement of FRET efficiency.",

author = "Taeseok Oh and Choi, {Jae Young} and Heller, {Michael J.}",

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AU - Choi, Jae Young

AU - Heller, Michael J.

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PY - 2016/4/21

Y1 - 2016/4/21

N2 - Dimerization and resultant quenching of donor and acceptor dyes conjugated on DNA causes loss of fluorescent resonant energy transfer (FRET) efficiency. However, when complexed with surfactants and divalent metal ions, sheathing effects insulate and shield the DNA structures, reducing dimerization and quenching which leads to significant enhancement of FRET efficiency.

AB - Dimerization and resultant quenching of donor and acceptor dyes conjugated on DNA causes loss of fluorescent resonant energy transfer (FRET) efficiency. However, when complexed with surfactants and divalent metal ions, sheathing effects insulate and shield the DNA structures, reducing dimerization and quenching which leads to significant enhancement of FRET efficiency.

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Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions

Abstract

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