Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions

Taeseok Oh, Jae Young Choi, Michael (Mike) Heller

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Dimerization and resultant quenching of donor and acceptor dyes conjugated on DNA causes loss of fluorescent resonant energy transfer (FRET) efficiency. However, when complexed with surfactants and divalent metal ions, sheathing effects insulate and shield the DNA structures, reducing dimerization and quenching which leads to significant enhancement of FRET efficiency.

Original languageEnglish (US)
Pages (from-to)2371-2375
Number of pages5
JournalAnalyst
Volume141
Issue number8
DOIs
StatePublished - Apr 21 2016
Externally publishedYes

Fingerprint

Dimerization
Energy Transfer
Surface-Active Agents
Energy transfer
Metal ions
surfactant
Quenching
Surface active agents
DNA
Metals
Ions
ion
metal
energy
shield
dye
Coloring Agents
Dyes
effect
loss

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions. / Oh, Taeseok; Choi, Jae Young; Heller, Michael (Mike).

In: Analyst, Vol. 141, No. 8, 21.04.2016, p. 2371-2375.

Research output: Contribution to journalArticle

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