Enhanced delivery of molecules into cells using laser-induced stress waves

Kunio Awazu, Steven L. Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

This study investigated the ability of laser-induced stress waves to enhance delivery of molecules into cells. Our experiment involved irradiating a thin layer of gel containing India ink at the tip of a capillary tube containing cells. The laser pulses (10 ns, 532 nm) deposited energy in the gel layer and generated intense stress waves which propagated into the capillary tube and affected the cells. The gel surface of irradiation was open to the air therefore the boundary condition was a free surface. The cell medium contained fluorescein attached to dextran of various molecular weights. After repeated laser pulses, the cells were collected on a glass slide and observed by fluorescence microscopy. Irradiated cells were observed to have taken up dye relative to unirradiated controls. The results indicate the ability of stress waves to enhance the uptake of large molecular weight reagents by cells.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsSteven L. Jacques
Pages167-172
Number of pages6
StatePublished - Jan 1 1996
EventLaser-Tissue Interaction VII - San Jose, CA, USA
Duration: Jan 29 1996Feb 1 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2681

Other

OtherLaser-Tissue Interaction VII
CitySan Jose, CA, USA
Period1/29/962/1/96

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Awazu, K., & Jacques, S. L. (1996). Enhanced delivery of molecules into cells using laser-induced stress waves. In S. L. Jacques (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 167-172). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2681).