Enhanced binding of the hypoxic cell marker [3H]fluoromisonidazole in ischemic myocardium

G. V. Martin, J. H. Caldwell, J. S. Rasey, Z. Grunbaum, M. Cerqueira, K. A. Krohn

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58 Scopus citations

Abstract

The 2-nitroimidazole fluoromisonidazole is metabolically trapped in viable hypoxic cells in inverse proportion to PO2. This attribute suggests that [18F]fluoromisonidazole may be useful for imaging hypoxic tissue using positron emission tomography. To examine this potential, we studied the pharmacokinetics and biodistribution of [3H]fluoromisonidazole in six open chest dogs. In two normal dogs, plasma and urine samples were collected over a 4-hr period following i.v. injection of the drug. In four animals, regional myocardial ischemia was produced 2 hr prior to drug injection by occlusion of the circumflex coronary artery and maintained during the 4-hr sampling period. In all animals, postmortem samples of myocardium and other organs were obtained and tissue, plasma, and urine tritium activity were determined by liquid scintillation counting. In areas of reduced flow, [3H]fluoromisonidazole accumulated in myocardium in inverse proportion to myocardial blood flow mesured by microspheres, indicating enhanced binding in hypoxic tissues. Maximum tissue concentrations in ischemic myocardium were two- to three-fold greater than in normal myocardium and plasma. Plasma clearance data indicate the drug is rapidly distributed into the total-body water, clears from the body with a half-life of 275 ± 50 min, and undergoes minimal metabolism by 4 hr. We conclude [18F]fluoromisonidazole may be a suitable agent for radionuclide imaging of hypoxic myocardium.

Original languageEnglish (US)
Pages (from-to)194-201
Number of pages8
JournalJournal of Nuclear Medicine
Volume30
Issue number2
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Martin, G. V., Caldwell, J. H., Rasey, J. S., Grunbaum, Z., Cerqueira, M., & Krohn, K. A. (1989). Enhanced binding of the hypoxic cell marker [3H]fluoromisonidazole in ischemic myocardium. Journal of Nuclear Medicine, 30(2), 194-201.