Enhanced binding of 3H-arginine8-vasopressin in the Brattleboro rat

L. M. Shewey, D. M. Dorsa

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Specific binding sites for 3[H]-arginine8-vasopressin (AVP) were characterized using membrane preparations of liver, renal medulla and brain (septal) tissue of heterozygous (HE) and homozygous (HO) Brattleboro (BB) rats. Measurement of binding sites indicated that significantly greater numbers of AVP receptors are present in the liver and septum of HO-BB rats. Similar numbers of AVP receptors were present in renal medullary tissue from HO-BB and HE-BB rats. Higher equilibrium dissociation constants were measured in the HO-BB septal tissue indicating a lower affinity of the brain receptor for 3[H]-AVP than in heterozygotes. No significant differences in AVP receptor affinity were noted in liver or kidney tissue. It is concluded that "up-regulation" of AVP receptor number and, in the brain, alterations in AVP receptor affinity may occur in the absence of endogenous AVP.

Original languageEnglish (US)
Pages (from-to)701-704
Number of pages4
JournalPeptides
Volume7
Issue number4
DOIs
StatePublished - Jan 1 1986

Keywords

  • Brain
  • Brattleboro rats
  • Receptors
  • Up-regulation
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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