Engraftment potential of different sources of human hematopoietic progenitor cells in BNX mice

Curtis W. Turner, Andrew M. Yeager, Edmund K. Waller, John R. Wingard, William Fleming

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Human hematopoietic progenitor cells (HPCs) from mobilized peripheral blood mononuclear cells (PBMCs), adult bone marrow (ABM), and fetal bone marrow (FBM) were evaluated for their ability to produce multilineage human hematopoietic engraftment in vivo. Sublethally irradiated BNX (beige/nude/xid) mice were injected with either unfractionated cells or CD34+ cells purified from these sources. The presence of human cells in the mouse PB, BM, and spleen was evaluated by flow cytometry at either 6 to 8 weeks or 6 months postinjection. Recipients with ≥1% human cells in any of these tissues were considered chimeric. Of 26 mice injected with FBM, 4 showed up to 73% human cells in the BM or spleen at 6 months. The phenotypes of these cells included CD13/33+ myelomonocytic cells (38%), CD19+ B cells (67%), and CD34+ progenitor cells (28%). In contrast, ABM gave rise to a mean of 5% human cells in the PB in 2 of 42 (4%) recipients at 6 to 8 weeks. These circulating human cells were predominately CD3+, whereas CD13/33+ and CD34+ cells were detected in the BM for up to 6 months. A total of 18% of mice injected with PBMCs showed a mean of 36% human cells in the PB. Both the BM and spleens of PBMC-injected mice contained CD3+ cells in a proportion similar to that observed in the PB. These CD3+ cells were phenotypically mature CD4+,CD8- or CD4-,CD8+ T cells and coexpressed a variety of Vβ T- cell receptor (TCR) genes. The percentage of CD3+ cells in the circulation of chimeric recipients injected with either FBM, ABM, or PBMCs correlated well with the input CD3+ cell dose for each of these HPC sources (r = .99). The high levels of engraftment of CD3+ cells in recipients of PBMCs and the long-term multilineage engraftment of FBM recipients have important implications for developing strategies to study the regulation of these human cells in vivo.

Original languageEnglish (US)
Pages (from-to)3237-3244
Number of pages8
JournalBlood
Volume87
Issue number8
StatePublished - Apr 15 1996
Externally publishedYes

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Hematopoietic Stem Cells
Nude Mice
Cells
Bone
Blood
Bone Marrow
Blood Cells
T-cells
Flow cytometry
Spleen
T-Cell Antigen Receptor
Genes
Tissue
T-Cell Receptor Genes

ASJC Scopus subject areas

  • Hematology

Cite this

Turner, C. W., Yeager, A. M., Waller, E. K., Wingard, J. R., & Fleming, W. (1996). Engraftment potential of different sources of human hematopoietic progenitor cells in BNX mice. Blood, 87(8), 3237-3244.

Engraftment potential of different sources of human hematopoietic progenitor cells in BNX mice. / Turner, Curtis W.; Yeager, Andrew M.; Waller, Edmund K.; Wingard, John R.; Fleming, William.

In: Blood, Vol. 87, No. 8, 15.04.1996, p. 3237-3244.

Research output: Contribution to journalArticle

Turner, CW, Yeager, AM, Waller, EK, Wingard, JR & Fleming, W 1996, 'Engraftment potential of different sources of human hematopoietic progenitor cells in BNX mice', Blood, vol. 87, no. 8, pp. 3237-3244.
Turner CW, Yeager AM, Waller EK, Wingard JR, Fleming W. Engraftment potential of different sources of human hematopoietic progenitor cells in BNX mice. Blood. 1996 Apr 15;87(8):3237-3244.
Turner, Curtis W. ; Yeager, Andrew M. ; Waller, Edmund K. ; Wingard, John R. ; Fleming, William. / Engraftment potential of different sources of human hematopoietic progenitor cells in BNX mice. In: Blood. 1996 ; Vol. 87, No. 8. pp. 3237-3244.
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abstract = "Human hematopoietic progenitor cells (HPCs) from mobilized peripheral blood mononuclear cells (PBMCs), adult bone marrow (ABM), and fetal bone marrow (FBM) were evaluated for their ability to produce multilineage human hematopoietic engraftment in vivo. Sublethally irradiated BNX (beige/nude/xid) mice were injected with either unfractionated cells or CD34+ cells purified from these sources. The presence of human cells in the mouse PB, BM, and spleen was evaluated by flow cytometry at either 6 to 8 weeks or 6 months postinjection. Recipients with ≥1{\%} human cells in any of these tissues were considered chimeric. Of 26 mice injected with FBM, 4 showed up to 73{\%} human cells in the BM or spleen at 6 months. The phenotypes of these cells included CD13/33+ myelomonocytic cells (38{\%}), CD19+ B cells (67{\%}), and CD34+ progenitor cells (28{\%}). In contrast, ABM gave rise to a mean of 5{\%} human cells in the PB in 2 of 42 (4{\%}) recipients at 6 to 8 weeks. These circulating human cells were predominately CD3+, whereas CD13/33+ and CD34+ cells were detected in the BM for up to 6 months. A total of 18{\%} of mice injected with PBMCs showed a mean of 36{\%} human cells in the PB. Both the BM and spleens of PBMC-injected mice contained CD3+ cells in a proportion similar to that observed in the PB. These CD3+ cells were phenotypically mature CD4+,CD8- or CD4-,CD8+ T cells and coexpressed a variety of Vβ T- cell receptor (TCR) genes. The percentage of CD3+ cells in the circulation of chimeric recipients injected with either FBM, ABM, or PBMCs correlated well with the input CD3+ cell dose for each of these HPC sources (r = .99). The high levels of engraftment of CD3+ cells in recipients of PBMCs and the long-term multilineage engraftment of FBM recipients have important implications for developing strategies to study the regulation of these human cells in vivo.",
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