Engineering Microvascular Networks in LED Light-cured Cell-Laden Hydrogels

Nelson Monteiro, Wenting He, Cristiane Miranda Franca, Avathamsa Athirasala, Luiz Bertassoni

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The success of tissue engineering inevitably depends on the fabrication of tissue constructs that can be vascularized and that anastomose with the host vasculature. In this report we studied the effects of light-emitting diode (LED) photopolymerized gelatin methacryloyl hydrogels (GelMA), encapsulated with stem cells from the apical papilla (SCAP) and human umbilical vein endothelial cells (HUVECs), in promoting vasculature network formation as a function of hydrogel physical and mechanical properties, as well as total cell density. Lithium acylphosphinate (LAP) was used as the photoinitiator in concentrations of 0.05, 0.075, 0.1% (w/v). GelMA hydrogel precursors of 5% (w/v) were encapsulated with co-cultures of SCAPs and HUVECs at different cell densities (1x, 5x and 10x106 cells/ml) and photocrosslinked for 5 s. Results suggested that the compressive modulus of GelMA hydrogels increased as a function of LAP concentration, and had a maximum stiffness of 3.2 kPa. GelMA hydrogels photopolymerized using 0.05 or 0.075% LAP, which had an average of 1.5 and 1.6 kPa of elastic modulus respectively, had the most efficient vasculature formation after 5 days, and these results were further enhanced when the highest cell density (10x106 cells/ml) was used. Immunofluorescence images showed that SCAP cells spread in close contact with endothelial networks and expressed alpha smooth muscle actin (αSMA), which is suggestive of their differentiation into pericyte-like cells. αSMA expression was also apparently higher in hydrogels polymerized with 0.05% LAP and 10x106 cells/ml. In conclusion, photopolymerization of GelMA hydrogels using an LED-light source can be an effective method for potential chair-side/in-situ procedures for engineering of vascularized tissue constructs in regenerative medicine.

Original languageEnglish (US)
JournalACS Biomaterials Science and Engineering
DOIs
StateAccepted/In press - Apr 27 2018

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Hydrogels
Light emitting diodes
Gelatin
Lithium
Hydrogel
Endothelial cells
Stem cells
Muscle
Actins
Tissue
Photopolymerization
Tissue engineering
Cell culture
Light sources
Physical properties
Elastic moduli
Stiffness
Fabrication
Mechanical properties

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Engineering Microvascular Networks in LED Light-cured Cell-Laden Hydrogels. / Monteiro, Nelson; He, Wenting; Miranda Franca, Cristiane; Athirasala, Avathamsa; Bertassoni, Luiz.

In: ACS Biomaterials Science and Engineering, 27.04.2018.

Research output: Contribution to journalArticle

Monteiro, Nelson ; He, Wenting ; Miranda Franca, Cristiane ; Athirasala, Avathamsa ; Bertassoni, Luiz. / Engineering Microvascular Networks in LED Light-cured Cell-Laden Hydrogels. In: ACS Biomaterials Science and Engineering. 2018.
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abstract = "The success of tissue engineering inevitably depends on the fabrication of tissue constructs that can be vascularized and that anastomose with the host vasculature. In this report we studied the effects of light-emitting diode (LED) photopolymerized gelatin methacryloyl hydrogels (GelMA), encapsulated with stem cells from the apical papilla (SCAP) and human umbilical vein endothelial cells (HUVECs), in promoting vasculature network formation as a function of hydrogel physical and mechanical properties, as well as total cell density. Lithium acylphosphinate (LAP) was used as the photoinitiator in concentrations of 0.05, 0.075, 0.1{\%} (w/v). GelMA hydrogel precursors of 5{\%} (w/v) were encapsulated with co-cultures of SCAPs and HUVECs at different cell densities (1x, 5x and 10x106 cells/ml) and photocrosslinked for 5 s. Results suggested that the compressive modulus of GelMA hydrogels increased as a function of LAP concentration, and had a maximum stiffness of 3.2 kPa. GelMA hydrogels photopolymerized using 0.05 or 0.075{\%} LAP, which had an average of 1.5 and 1.6 kPa of elastic modulus respectively, had the most efficient vasculature formation after 5 days, and these results were further enhanced when the highest cell density (10x106 cells/ml) was used. Immunofluorescence images showed that SCAP cells spread in close contact with endothelial networks and expressed alpha smooth muscle actin (αSMA), which is suggestive of their differentiation into pericyte-like cells. αSMA expression was also apparently higher in hydrogels polymerized with 0.05{\%} LAP and 10x106 cells/ml. In conclusion, photopolymerization of GelMA hydrogels using an LED-light source can be an effective method for potential chair-side/in-situ procedures for engineering of vascularized tissue constructs in regenerative medicine.",
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