Engineering K+ channels using semisynthesis

Alexander G. Komarov, Corey A. Costantino, Francis Valiyaveetil

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Potassium channels conduct K+ ions selectively and at very high rates. Central to the function of K+ channels is a structural unit called the selectivity filter. In the selectivity filter, a row of four K + binding sites are created using mainly the backbone carbonyl oxygen atoms. Due to the involvement of the protein backbone, site-directed mutagenesis is of limited utility in investigating the selectivity filter. In order to overcome this limitation, we have developed a semisynthetic approach, which permits the use of chemical synthesis to manipulate the selectivity filter. In this chapter, we describe the protocols that we have developed for the semisynthesis of the K+ channel, KcsA. We anticipate that the protocols described in this chapter will also be applicable for the semisynthesis of other integral membrane proteins of interest.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages3-17
Number of pages15
Volume995
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume995
ISSN (Print)10643745

Fingerprint

Potassium Channels
Site-Directed Mutagenesis
Membrane Proteins
Binding Sites
Ions
Oxygen
Proteins

Keywords

  • K channels
  • Membrane proteins
  • Semisynthesis
  • Solid-phase peptide synthesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Komarov, A. G., Costantino, C. A., & Valiyaveetil, F. (2013). Engineering K+ channels using semisynthesis. In Methods in Molecular Biology (Vol. 995, pp. 3-17). (Methods in Molecular Biology; Vol. 995). https://doi.org/10.1007/978-1-62703-345-9_1

Engineering K+ channels using semisynthesis. / Komarov, Alexander G.; Costantino, Corey A.; Valiyaveetil, Francis.

Methods in Molecular Biology. Vol. 995 2013. p. 3-17 (Methods in Molecular Biology; Vol. 995).

Research output: Chapter in Book/Report/Conference proceedingChapter

Komarov, AG, Costantino, CA & Valiyaveetil, F 2013, Engineering K+ channels using semisynthesis. in Methods in Molecular Biology. vol. 995, Methods in Molecular Biology, vol. 995, pp. 3-17. https://doi.org/10.1007/978-1-62703-345-9_1
Komarov AG, Costantino CA, Valiyaveetil F. Engineering K+ channels using semisynthesis. In Methods in Molecular Biology. Vol. 995. 2013. p. 3-17. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-345-9_1
Komarov, Alexander G. ; Costantino, Corey A. ; Valiyaveetil, Francis. / Engineering K+ channels using semisynthesis. Methods in Molecular Biology. Vol. 995 2013. pp. 3-17 (Methods in Molecular Biology).
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