Endotoxin tolerance diminishes certain antiinflammatory effects of endotoxin

James (Jim) Rosenbaum, Kaija T. Hartiala, Edward L. Howes, Ira M. Goldstein

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Endotoxin (bacterial lipopolysaccharide, LPS) is paradoxically both inflammatory and antiinflammatory. A single intravenous injection of 100 μg Escherichia coli LPS markedly inhibits the inflammatory changes associated with cutaneous reversed passive Arthus (RPA) reactions in New Zealand white rabbits. Polymorphonuclear (PMN) leukocytes from LPS-treated rabbits exhibit diminished responsiveness in vitro to complement (C5) -derived peptides. Repeated injections of LPS render animals "tolerant", that is, refractory to the toxic and inflammatory efects of LPS. We examined whether tolerance would enhance the ability of LPS to inhibit inflammation not attributable to LPS. Surprisingly, as compared with rabbits receiving a single dose of LPS, tolerant rabbits demonstrated greater inflammatory changes (i.e., PMN exudation, vascular permeability) associated with RPA reactions. PMNs from LPS-tolerant rabbits responded in vitro to C5-derived peptides significantly more than PMNs from rabbits that received a single dose of LPS. We speculate that some antiinflammatory effects of LPS require the toxic or inflammatory effects of LPS itself. These observations might relate to the limited efficacy of fever therapy and the variable effects of gram-negative sepsis on functions of human PMNs.

Original languageEnglish (US)
Pages (from-to)297-308
Number of pages12
JournalInflammation
Volume9
Issue number3
DOIs
StatePublished - Sep 1985
Externally publishedYes

Fingerprint

Endotoxins
Lipopolysaccharides
Anti-Inflammatory Agents
Rabbits
Arthus Reaction
Poisons
Complement C5
Induced Hyperthermia
Capillary Permeability
Intravenous Injections
Sepsis
Neutrophils
Escherichia coli
Inflammation
Skin
Injections

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

Endotoxin tolerance diminishes certain antiinflammatory effects of endotoxin. / Rosenbaum, James (Jim); Hartiala, Kaija T.; Howes, Edward L.; Goldstein, Ira M.

In: Inflammation, Vol. 9, No. 3, 09.1985, p. 297-308.

Research output: Contribution to journalArticle

Rosenbaum, James (Jim) ; Hartiala, Kaija T. ; Howes, Edward L. ; Goldstein, Ira M. / Endotoxin tolerance diminishes certain antiinflammatory effects of endotoxin. In: Inflammation. 1985 ; Vol. 9, No. 3. pp. 297-308.
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