Endotoxin-induced uveitis (EIU) in mice with IL-1 receptor and TNF receptor genes deleted

Stephen Planck, Y. B. Han, J. M. Park, B. Brito, L. O'Rourke, X. N. Huang, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

Abstract

Purpose: To determine the role of interleukin-1 (IL-1) and tumor necrosis factor a (TNF) in the pathogenesis of EIU. Methods: 250 ng E.coli endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I (IL-1R1-/-), TNF receptors p55 and p75 (TNFRSS/75-/-), IL-IR1-/-/TNFR55-/-) or congenic controls (Immunex, Seattle, WA).. Eyes were harvested after 24 h for histologie analysis and collection of aqueous humor or after 3 h for RT-PCR analysis. IL-6 activity in aqueous humor was measured with a B9 cell bioassay. Results: No significant difference in the number of infiltrating cells was found in TNFR55/75-/- mice compared to controls in any of 4 separate experiments or in the combined data (total n= 38). The number of infiltrating cells was significantly reduced in 2 experiments with IL-lRl-/- mice (p-/- /TNFRSS''' mice had a reduction in infiltrating cells in 2 of 3 experiments and in the combined data (all p-/- mice, but were reduced in 1 of 2 experiments with IL-1R1-/- mice (n=8, p=0.02) and in 1 experiment with IL-1R1-/-/TNFR55-/- mice (n=12, p=0.01). Conclusions IL-1 appears to have a more pivotal role in EIU than TNF, although neither cytokine is essential. Deletion of receptors for both cytokines has the most pronounced effect which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant. NIH grants: EYO6468, EYO6477, EY10572.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - 1997

Fingerprint

Interleukin-1 Receptors
Tumor Necrosis Factor Receptors
Uveitis
Endotoxins
Genes
Aqueous Humor
Interleukin-1
Interleukin-1 Type I Receptors
Tumor Necrosis Factor-alpha
Cell Count
Cytokines
Cytokine Receptors
Organized Financing
Biological Assay
Interleukin-6
Escherichia coli
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Endotoxin-induced uveitis (EIU) in mice with IL-1 receptor and TNF receptor genes deleted. / Planck, Stephen; Han, Y. B.; Park, J. M.; Brito, B.; O'Rourke, L.; Huang, X. N.; Rosenbaum, James (Jim).

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 1997.

Research output: Contribution to journalArticle

@article{b78767b0732041e29cfc7fc55de1d21e,
title = "Endotoxin-induced uveitis (EIU) in mice with IL-1 receptor and TNF receptor genes deleted",
abstract = "Purpose: To determine the role of interleukin-1 (IL-1) and tumor necrosis factor a (TNF) in the pathogenesis of EIU. Methods: 250 ng E.coli endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I (IL-1R1-/-), TNF receptors p55 and p75 (TNFRSS/75-/-), IL-IR1-/-/TNFR55-/-) or congenic controls (Immunex, Seattle, WA).. Eyes were harvested after 24 h for histologie analysis and collection of aqueous humor or after 3 h for RT-PCR analysis. IL-6 activity in aqueous humor was measured with a B9 cell bioassay. Results: No significant difference in the number of infiltrating cells was found in TNFR55/75-/- mice compared to controls in any of 4 separate experiments or in the combined data (total n= 38). The number of infiltrating cells was significantly reduced in 2 experiments with IL-lRl-/- mice (p-/- /TNFRSS''' mice had a reduction in infiltrating cells in 2 of 3 experiments and in the combined data (all p-/- mice, but were reduced in 1 of 2 experiments with IL-1R1-/- mice (n=8, p=0.02) and in 1 experiment with IL-1R1-/-/TNFR55-/- mice (n=12, p=0.01). Conclusions IL-1 appears to have a more pivotal role in EIU than TNF, although neither cytokine is essential. Deletion of receptors for both cytokines has the most pronounced effect which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant. NIH grants: EYO6468, EYO6477, EY10572.",
author = "Stephen Planck and Han, {Y. B.} and Park, {J. M.} and B. Brito and L. O'Rourke and Huang, {X. N.} and Rosenbaum, {James (Jim)}",
year = "1997",
language = "English (US)",
volume = "38",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "4",

}

TY - JOUR

T1 - Endotoxin-induced uveitis (EIU) in mice with IL-1 receptor and TNF receptor genes deleted

AU - Planck, Stephen

AU - Han, Y. B.

AU - Park, J. M.

AU - Brito, B.

AU - O'Rourke, L.

AU - Huang, X. N.

AU - Rosenbaum, James (Jim)

PY - 1997

Y1 - 1997

N2 - Purpose: To determine the role of interleukin-1 (IL-1) and tumor necrosis factor a (TNF) in the pathogenesis of EIU. Methods: 250 ng E.coli endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I (IL-1R1-/-), TNF receptors p55 and p75 (TNFRSS/75-/-), IL-IR1-/-/TNFR55-/-) or congenic controls (Immunex, Seattle, WA).. Eyes were harvested after 24 h for histologie analysis and collection of aqueous humor or after 3 h for RT-PCR analysis. IL-6 activity in aqueous humor was measured with a B9 cell bioassay. Results: No significant difference in the number of infiltrating cells was found in TNFR55/75-/- mice compared to controls in any of 4 separate experiments or in the combined data (total n= 38). The number of infiltrating cells was significantly reduced in 2 experiments with IL-lRl-/- mice (p-/- /TNFRSS''' mice had a reduction in infiltrating cells in 2 of 3 experiments and in the combined data (all p-/- mice, but were reduced in 1 of 2 experiments with IL-1R1-/- mice (n=8, p=0.02) and in 1 experiment with IL-1R1-/-/TNFR55-/- mice (n=12, p=0.01). Conclusions IL-1 appears to have a more pivotal role in EIU than TNF, although neither cytokine is essential. Deletion of receptors for both cytokines has the most pronounced effect which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant. NIH grants: EYO6468, EYO6477, EY10572.

AB - Purpose: To determine the role of interleukin-1 (IL-1) and tumor necrosis factor a (TNF) in the pathogenesis of EIU. Methods: 250 ng E.coli endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I (IL-1R1-/-), TNF receptors p55 and p75 (TNFRSS/75-/-), IL-IR1-/-/TNFR55-/-) or congenic controls (Immunex, Seattle, WA).. Eyes were harvested after 24 h for histologie analysis and collection of aqueous humor or after 3 h for RT-PCR analysis. IL-6 activity in aqueous humor was measured with a B9 cell bioassay. Results: No significant difference in the number of infiltrating cells was found in TNFR55/75-/- mice compared to controls in any of 4 separate experiments or in the combined data (total n= 38). The number of infiltrating cells was significantly reduced in 2 experiments with IL-lRl-/- mice (p-/- /TNFRSS''' mice had a reduction in infiltrating cells in 2 of 3 experiments and in the combined data (all p-/- mice, but were reduced in 1 of 2 experiments with IL-1R1-/- mice (n=8, p=0.02) and in 1 experiment with IL-1R1-/-/TNFR55-/- mice (n=12, p=0.01). Conclusions IL-1 appears to have a more pivotal role in EIU than TNF, although neither cytokine is essential. Deletion of receptors for both cytokines has the most pronounced effect which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant. NIH grants: EYO6468, EYO6477, EY10572.

UR - http://www.scopus.com/inward/record.url?scp=33749089469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749089469&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749089469

VL - 38

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 4

ER -