Endothelin transfer and endothelin effects on water transfer in human fetal membranes

A. W. Eis, M. D. Mitchell, Leslie Myatt

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Although endothelin-1 is synthesized by human amnion, its physiologic role and its ability to be transferred to myometrium for oxytocic action remain unclear. We investigated the transfer of endothelin-1 itself and the effects of endothelin-1 on the transfer of tritiated water across human amnion or amnion/chorion/decidua in vitro with an Ussing chamber technique. Permeability coefficients (mean ± standard error) for 3H2O across amnion/chorion/decidua in the fetal to maternal direction were 1.26 ± 0.21 and 1.25 ± 0.10 x 10-4 cm/second (N = 6) and in the maternal to fetal direction 0.90 ± 0.07 and 0.98 ± 0.15 x 10-4 cm/second (N = 5) in the absence or presence, respectively, of 10-9 mol/L endothelin-1 in the fetal reservoir. Comparable values were found in either direction with 10-9 mol/L endothelin-1 in the maternal reservoir. Apparent permeability coefficients for 125I endothelin-1 in the fetal to maternal direction were 3.34 ± 0.79 and 2.43 ± 0.68 x 10-5 cm/second (N = 5) for amnion or amnion/chorion/decidua. However, appreciable trapping of 125I endothelin-1 by the fetal membranes was apparent. Endothelin-1 does not appear to affect water movement across the human fetal membranes, and only a small proportion of endothelin-1 itself is transferred across the membranes.

Original languageEnglish (US)
Pages (from-to)411-415
Number of pages5
JournalObstetrics and Gynecology
Volume79
Issue number3
StatePublished - 1992
Externally publishedYes

Fingerprint

Extraembryonic Membranes
Endothelins
Endothelin-1
Amnion
Water
Chorion
Decidua
Mothers
Permeability
Oxytocics
Water Movements
Myometrium
Membranes

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Endothelin transfer and endothelin effects on water transfer in human fetal membranes. / Eis, A. W.; Mitchell, M. D.; Myatt, Leslie.

In: Obstetrics and Gynecology, Vol. 79, No. 3, 1992, p. 411-415.

Research output: Contribution to journalArticle

@article{732a734ebb504f5bad76a7ca154cfdce,
title = "Endothelin transfer and endothelin effects on water transfer in human fetal membranes",
abstract = "Although endothelin-1 is synthesized by human amnion, its physiologic role and its ability to be transferred to myometrium for oxytocic action remain unclear. We investigated the transfer of endothelin-1 itself and the effects of endothelin-1 on the transfer of tritiated water across human amnion or amnion/chorion/decidua in vitro with an Ussing chamber technique. Permeability coefficients (mean ± standard error) for 3H2O across amnion/chorion/decidua in the fetal to maternal direction were 1.26 ± 0.21 and 1.25 ± 0.10 x 10-4 cm/second (N = 6) and in the maternal to fetal direction 0.90 ± 0.07 and 0.98 ± 0.15 x 10-4 cm/second (N = 5) in the absence or presence, respectively, of 10-9 mol/L endothelin-1 in the fetal reservoir. Comparable values were found in either direction with 10-9 mol/L endothelin-1 in the maternal reservoir. Apparent permeability coefficients for 125I endothelin-1 in the fetal to maternal direction were 3.34 ± 0.79 and 2.43 ± 0.68 x 10-5 cm/second (N = 5) for amnion or amnion/chorion/decidua. However, appreciable trapping of 125I endothelin-1 by the fetal membranes was apparent. Endothelin-1 does not appear to affect water movement across the human fetal membranes, and only a small proportion of endothelin-1 itself is transferred across the membranes.",
author = "Eis, {A. W.} and Mitchell, {M. D.} and Leslie Myatt",
year = "1992",
language = "English (US)",
volume = "79",
pages = "411--415",
journal = "Obstetrics and Gynecology",
issn = "0029-7844",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Endothelin transfer and endothelin effects on water transfer in human fetal membranes

AU - Eis, A. W.

AU - Mitchell, M. D.

AU - Myatt, Leslie

PY - 1992

Y1 - 1992

N2 - Although endothelin-1 is synthesized by human amnion, its physiologic role and its ability to be transferred to myometrium for oxytocic action remain unclear. We investigated the transfer of endothelin-1 itself and the effects of endothelin-1 on the transfer of tritiated water across human amnion or amnion/chorion/decidua in vitro with an Ussing chamber technique. Permeability coefficients (mean ± standard error) for 3H2O across amnion/chorion/decidua in the fetal to maternal direction were 1.26 ± 0.21 and 1.25 ± 0.10 x 10-4 cm/second (N = 6) and in the maternal to fetal direction 0.90 ± 0.07 and 0.98 ± 0.15 x 10-4 cm/second (N = 5) in the absence or presence, respectively, of 10-9 mol/L endothelin-1 in the fetal reservoir. Comparable values were found in either direction with 10-9 mol/L endothelin-1 in the maternal reservoir. Apparent permeability coefficients for 125I endothelin-1 in the fetal to maternal direction were 3.34 ± 0.79 and 2.43 ± 0.68 x 10-5 cm/second (N = 5) for amnion or amnion/chorion/decidua. However, appreciable trapping of 125I endothelin-1 by the fetal membranes was apparent. Endothelin-1 does not appear to affect water movement across the human fetal membranes, and only a small proportion of endothelin-1 itself is transferred across the membranes.

AB - Although endothelin-1 is synthesized by human amnion, its physiologic role and its ability to be transferred to myometrium for oxytocic action remain unclear. We investigated the transfer of endothelin-1 itself and the effects of endothelin-1 on the transfer of tritiated water across human amnion or amnion/chorion/decidua in vitro with an Ussing chamber technique. Permeability coefficients (mean ± standard error) for 3H2O across amnion/chorion/decidua in the fetal to maternal direction were 1.26 ± 0.21 and 1.25 ± 0.10 x 10-4 cm/second (N = 6) and in the maternal to fetal direction 0.90 ± 0.07 and 0.98 ± 0.15 x 10-4 cm/second (N = 5) in the absence or presence, respectively, of 10-9 mol/L endothelin-1 in the fetal reservoir. Comparable values were found in either direction with 10-9 mol/L endothelin-1 in the maternal reservoir. Apparent permeability coefficients for 125I endothelin-1 in the fetal to maternal direction were 3.34 ± 0.79 and 2.43 ± 0.68 x 10-5 cm/second (N = 5) for amnion or amnion/chorion/decidua. However, appreciable trapping of 125I endothelin-1 by the fetal membranes was apparent. Endothelin-1 does not appear to affect water movement across the human fetal membranes, and only a small proportion of endothelin-1 itself is transferred across the membranes.

UR - http://www.scopus.com/inward/record.url?scp=0026517348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026517348&partnerID=8YFLogxK

M3 - Article

VL - 79

SP - 411

EP - 415

JO - Obstetrics and Gynecology

JF - Obstetrics and Gynecology

SN - 0029-7844

IS - 3

ER -