Endothelin transfer and endothelin effects on water transfer in human fetal membranes

A. W. Eis, M. D. Mitchell, L. Myatt

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Although endothelin-1 is synthesized by human amnion, its physiologic role and its ability to be transferred to myometrium for oxytocic action remain unclear. We investigated the transfer of endothelin-1 itself and the effects of endothelin-1 on the transfer of tritiated water across human amnion or amnion/chorion/decidua in vitro with an Ussing chamber technique. Permeability coefficients (mean ± standard error) for 3H2O across amnion/chorion/decidua in the fetal to maternal direction were 1.26 ± 0.21 and 1.25 ± 0.10 x 10-4 cm/second (N = 6) and in the maternal to fetal direction 0.90 ± 0.07 and 0.98 ± 0.15 x 10-4 cm/second (N = 5) in the absence or presence, respectively, of 10-9 mol/L endothelin-1 in the fetal reservoir. Comparable values were found in either direction with 10-9 mol/L endothelin-1 in the maternal reservoir. Apparent permeability coefficients for 125I endothelin-1 in the fetal to maternal direction were 3.34 ± 0.79 and 2.43 ± 0.68 x 10-5 cm/second (N = 5) for amnion or amnion/chorion/decidua. However, appreciable trapping of 125I endothelin-1 by the fetal membranes was apparent. Endothelin-1 does not appear to affect water movement across the human fetal membranes, and only a small proportion of endothelin-1 itself is transferred across the membranes.

Original languageEnglish (US)
Pages (from-to)411-415
Number of pages5
JournalObstetrics and gynecology
Issue number3
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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