Endothelin induces transcription of fos/jun family genes: A prominent role for calcium ion

David Pribnow, Leslie Muldoon, Mark Fajardo, Livia Theodor, Li Ye Stephen Chen, Bruce E. Magun

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The 21-amino acid mammalian peptide endothelin (ET) is a powerful vasoconstrictor, a mitogen for fibroblasts and vascular smooth muscle cells, and a potent effector for numerous tissues. Through extracellular interaction with G protein-coupled transmembrane receptors, ET stimulates intracellular second messenger events that in turn activate immediate early gene transcription. Using Northern blot hybridization and nuclear run-on analyses we examined the modulation of c-fos, fos-B, fra-1, c-jun, and jun-B gene transcripts in Rat-1 fibroblasts after ET treatment. Furthermore, we invested the role that intracellular Ca2+ transients played in effecting this gene regulation, using the intracellular Ca2+ chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′,-tetraacetic acid (BAPTA) to block Ca2+-dependent transcription. Our results demonstrate that ET rapidly effects increased RNA levels for all five fos/jun family genes investigated at least two of them by increasing gene transcription. Furthermore, our results argue that increased intracellular free Ca2+ is directly involved in the induction of these fos/jun family genes by ET. While mobilization of intracellular Ca2+ is not the only pathway to fos/jun gene induction used by ET, it is clearly a major component of the signaling apparatus that is set in motion by this potent effector.

Original languageEnglish (US)
Pages (from-to)1003-1012
Number of pages10
JournalMolecular Endocrinology
Volume6
Issue number7
StatePublished - Jul 1992

Fingerprint

jun Genes
Endothelins
Ions
Calcium
Fibroblasts
Genetic Transcription
fos Genes
Ethane
Immediate-Early Genes
Second Messenger Systems
Vasoconstrictor Agents
Chelating Agents
G-Protein-Coupled Receptors
Vascular Smooth Muscle
Mitogens
Northern Blotting
Genes
Smooth Muscle Myocytes
RNA
Amino Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Pribnow, D., Muldoon, L., Fajardo, M., Theodor, L., Chen, L. Y. S., & Magun, B. E. (1992). Endothelin induces transcription of fos/jun family genes: A prominent role for calcium ion. Molecular Endocrinology, 6(7), 1003-1012.

Endothelin induces transcription of fos/jun family genes : A prominent role for calcium ion. / Pribnow, David; Muldoon, Leslie; Fajardo, Mark; Theodor, Livia; Chen, Li Ye Stephen; Magun, Bruce E.

In: Molecular Endocrinology, Vol. 6, No. 7, 07.1992, p. 1003-1012.

Research output: Contribution to journalArticle

Pribnow, D, Muldoon, L, Fajardo, M, Theodor, L, Chen, LYS & Magun, BE 1992, 'Endothelin induces transcription of fos/jun family genes: A prominent role for calcium ion', Molecular Endocrinology, vol. 6, no. 7, pp. 1003-1012.
Pribnow, David ; Muldoon, Leslie ; Fajardo, Mark ; Theodor, Livia ; Chen, Li Ye Stephen ; Magun, Bruce E. / Endothelin induces transcription of fos/jun family genes : A prominent role for calcium ion. In: Molecular Endocrinology. 1992 ; Vol. 6, No. 7. pp. 1003-1012.
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