Endothelial cell, pericyte, and perivascular resident macrophage-type melanocyte interactions regulate cochlear intrastrial fluid-blood barrier permeability

Lingling Neng, Fei Zhang, Allan Kachelmeier, Xiao Shi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The integrity of the fluid-blood barrier in the stria vascularis is critical for maintaining inner ear homeostasis, especially for sustaining the endocochlear potential, an essential driving force for hearing function. However, the mechanisms that control intrastrial fluid-blood barrier permeability remain largely unknown. At the cellular level, the intrastrial fluid-blood barrier comprises cochlear microvascular endothelial cells connected to each other by tight junctions (TJs), an underlying basement membrane, and a second line of support consisting of cochlear pericytes and perivascular resident macrophage-type melanocytes. In this study, we use a newly established primary cell culture-based in vitro model to show that endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes interact to control intrastrial fluid-blood barrier permeability. When the endothelial cell monolayer was treated with pericyte - or perivascular resident macrophage-type melanocyte - conditioned media, the permeability of the endothelial cell monolayer was significantly reduced relative to an untreated endothelial cell monolayer. Further study has shown the pericytes and perivascular resident macrophage-type melanocytes to regulate TJ expression in the endothelial cell monolayer. The new cell culture-based in vitro model offers a unique opportunity to obtain information on the organ-specific characteristics of the cochlear blood/tissue barrier. Our finding demonstrates the importance of signaling among pericytes, endothelial cells, and perivascular resident macrophage-type melanocytes to the integrity of the intrastrial fluid-blood barrier.

Original languageEnglish (US)
Pages (from-to)175-185
Number of pages11
JournalJARO - Journal of the Association for Research in Otolaryngology
Volume14
Issue number2
DOIs
StatePublished - Apr 2013

Fingerprint

Pericytes
Melanocytes
Cochlea
Permeability
Endothelial Cells
Macrophages
Tight Junctions
Stria Vascularis
Primary Cell Culture
Inner Ear
Conditioned Culture Medium
Basement Membrane
Hearing
Homeostasis
Cell Culture Techniques

Keywords

  • endothelial cell
  • mouse cochlea
  • paracellular permeability
  • pericyte
  • perivascular resident macrophages-type melanocyte
  • primary cell line

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Sensory Systems

Cite this

@article{85b25f9ec2664fe9855a5586e1c1e1ed,
title = "Endothelial cell, pericyte, and perivascular resident macrophage-type melanocyte interactions regulate cochlear intrastrial fluid-blood barrier permeability",
abstract = "The integrity of the fluid-blood barrier in the stria vascularis is critical for maintaining inner ear homeostasis, especially for sustaining the endocochlear potential, an essential driving force for hearing function. However, the mechanisms that control intrastrial fluid-blood barrier permeability remain largely unknown. At the cellular level, the intrastrial fluid-blood barrier comprises cochlear microvascular endothelial cells connected to each other by tight junctions (TJs), an underlying basement membrane, and a second line of support consisting of cochlear pericytes and perivascular resident macrophage-type melanocytes. In this study, we use a newly established primary cell culture-based in vitro model to show that endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes interact to control intrastrial fluid-blood barrier permeability. When the endothelial cell monolayer was treated with pericyte - or perivascular resident macrophage-type melanocyte - conditioned media, the permeability of the endothelial cell monolayer was significantly reduced relative to an untreated endothelial cell monolayer. Further study has shown the pericytes and perivascular resident macrophage-type melanocytes to regulate TJ expression in the endothelial cell monolayer. The new cell culture-based in vitro model offers a unique opportunity to obtain information on the organ-specific characteristics of the cochlear blood/tissue barrier. Our finding demonstrates the importance of signaling among pericytes, endothelial cells, and perivascular resident macrophage-type melanocytes to the integrity of the intrastrial fluid-blood barrier.",
keywords = "endothelial cell, mouse cochlea, paracellular permeability, pericyte, perivascular resident macrophages-type melanocyte, primary cell line",
author = "Lingling Neng and Fei Zhang and Allan Kachelmeier and Xiao Shi",
year = "2013",
month = "4",
doi = "10.1007/s10162-012-0365-9",
language = "English (US)",
volume = "14",
pages = "175--185",
journal = "JARO - Journal of the Association for Research in Otolaryngology",
issn = "1525-3961",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Endothelial cell, pericyte, and perivascular resident macrophage-type melanocyte interactions regulate cochlear intrastrial fluid-blood barrier permeability

AU - Neng, Lingling

AU - Zhang, Fei

AU - Kachelmeier, Allan

AU - Shi, Xiao

PY - 2013/4

Y1 - 2013/4

N2 - The integrity of the fluid-blood barrier in the stria vascularis is critical for maintaining inner ear homeostasis, especially for sustaining the endocochlear potential, an essential driving force for hearing function. However, the mechanisms that control intrastrial fluid-blood barrier permeability remain largely unknown. At the cellular level, the intrastrial fluid-blood barrier comprises cochlear microvascular endothelial cells connected to each other by tight junctions (TJs), an underlying basement membrane, and a second line of support consisting of cochlear pericytes and perivascular resident macrophage-type melanocytes. In this study, we use a newly established primary cell culture-based in vitro model to show that endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes interact to control intrastrial fluid-blood barrier permeability. When the endothelial cell monolayer was treated with pericyte - or perivascular resident macrophage-type melanocyte - conditioned media, the permeability of the endothelial cell monolayer was significantly reduced relative to an untreated endothelial cell monolayer. Further study has shown the pericytes and perivascular resident macrophage-type melanocytes to regulate TJ expression in the endothelial cell monolayer. The new cell culture-based in vitro model offers a unique opportunity to obtain information on the organ-specific characteristics of the cochlear blood/tissue barrier. Our finding demonstrates the importance of signaling among pericytes, endothelial cells, and perivascular resident macrophage-type melanocytes to the integrity of the intrastrial fluid-blood barrier.

AB - The integrity of the fluid-blood barrier in the stria vascularis is critical for maintaining inner ear homeostasis, especially for sustaining the endocochlear potential, an essential driving force for hearing function. However, the mechanisms that control intrastrial fluid-blood barrier permeability remain largely unknown. At the cellular level, the intrastrial fluid-blood barrier comprises cochlear microvascular endothelial cells connected to each other by tight junctions (TJs), an underlying basement membrane, and a second line of support consisting of cochlear pericytes and perivascular resident macrophage-type melanocytes. In this study, we use a newly established primary cell culture-based in vitro model to show that endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes interact to control intrastrial fluid-blood barrier permeability. When the endothelial cell monolayer was treated with pericyte - or perivascular resident macrophage-type melanocyte - conditioned media, the permeability of the endothelial cell monolayer was significantly reduced relative to an untreated endothelial cell monolayer. Further study has shown the pericytes and perivascular resident macrophage-type melanocytes to regulate TJ expression in the endothelial cell monolayer. The new cell culture-based in vitro model offers a unique opportunity to obtain information on the organ-specific characteristics of the cochlear blood/tissue barrier. Our finding demonstrates the importance of signaling among pericytes, endothelial cells, and perivascular resident macrophage-type melanocytes to the integrity of the intrastrial fluid-blood barrier.

KW - endothelial cell

KW - mouse cochlea

KW - paracellular permeability

KW - pericyte

KW - perivascular resident macrophages-type melanocyte

KW - primary cell line

UR - http://www.scopus.com/inward/record.url?scp=84879606476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879606476&partnerID=8YFLogxK

U2 - 10.1007/s10162-012-0365-9

DO - 10.1007/s10162-012-0365-9

M3 - Article

VL - 14

SP - 175

EP - 185

JO - JARO - Journal of the Association for Research in Otolaryngology

JF - JARO - Journal of the Association for Research in Otolaryngology

SN - 1525-3961

IS - 2

ER -