TY - JOUR
T1 - Endothelial cell micropatterning
T2 - Methods, effects, and applications
AU - Anderson, Deirdre E.J.
AU - Hinds, Monica T.
N1 - Funding Information:
The authors gratefully acknowledge funding from the American Heart Association grant 09BGIA22 60384 and National Institutes of Health grants R01HL103728 and R01HL 095474.
PY - 2011/9
Y1 - 2011/9
N2 - The effects of flow on endothelial cells (ECs) have been widely examined for the ability of fluid shear stress to alter cell morphology and function; however, the effects of EC morphology without flow have only recently been observed. An increase in lithographic techniques in cell culture spurred a corresponding increase in research aiming to confine cell morphology. These studies lead to a better understanding of how morphology and cytoskeletal configuration affect the structure and function of the cells. This review examines EC micropatterning research by exploring both the many alternative methods used to alter EC morphology and the resulting changes in cellular shape and phenotype. Micropatterning induced changes in EC proliferation, apoptosis, cytoskeletal organization, mechanical properties, and cell functionality. Finally, the ways these cellular manipulation techniques have been applied to biomedical engineering research, including angiogenesis, cell migration, and tissue engineering, are discussed.
AB - The effects of flow on endothelial cells (ECs) have been widely examined for the ability of fluid shear stress to alter cell morphology and function; however, the effects of EC morphology without flow have only recently been observed. An increase in lithographic techniques in cell culture spurred a corresponding increase in research aiming to confine cell morphology. These studies lead to a better understanding of how morphology and cytoskeletal configuration affect the structure and function of the cells. This review examines EC micropatterning research by exploring both the many alternative methods used to alter EC morphology and the resulting changes in cellular shape and phenotype. Micropatterning induced changes in EC proliferation, apoptosis, cytoskeletal organization, mechanical properties, and cell functionality. Finally, the ways these cellular manipulation techniques have been applied to biomedical engineering research, including angiogenesis, cell migration, and tissue engineering, are discussed.
KW - Angiogenesis
KW - Cytoskeleton
KW - Endothelial cell
KW - Micropatterning
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=80051551702&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051551702&partnerID=8YFLogxK
U2 - 10.1007/s10439-011-0352-z
DO - 10.1007/s10439-011-0352-z
M3 - Article
C2 - 21761242
AN - SCOPUS:80051551702
SN - 0090-6964
VL - 39
SP - 2329
EP - 2345
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 9
ER -