Endothelial cell micropatterning: Methods, effects, and applications

Deirdre E.J. Anderson, Monica T. Hinds

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

The effects of flow on endothelial cells (ECs) have been widely examined for the ability of fluid shear stress to alter cell morphology and function; however, the effects of EC morphology without flow have only recently been observed. An increase in lithographic techniques in cell culture spurred a corresponding increase in research aiming to confine cell morphology. These studies lead to a better understanding of how morphology and cytoskeletal configuration affect the structure and function of the cells. This review examines EC micropatterning research by exploring both the many alternative methods used to alter EC morphology and the resulting changes in cellular shape and phenotype. Micropatterning induced changes in EC proliferation, apoptosis, cytoskeletal organization, mechanical properties, and cell functionality. Finally, the ways these cellular manipulation techniques have been applied to biomedical engineering research, including angiogenesis, cell migration, and tissue engineering, are discussed.

Original languageEnglish (US)
Pages (from-to)2329-2345
Number of pages17
JournalAnnals of Biomedical Engineering
Volume39
Issue number9
DOIs
StatePublished - Sep 2011

Keywords

  • Angiogenesis
  • Cytoskeleton
  • Endothelial cell
  • Micropatterning
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering

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