Endogenous truncated TrkB.T1 receptor regulates neuronal complexity and TrkB kinase receptor function in vivo

Laura Carim Todd, Kevin G. Bath, Gianluca Fulgenzi, Sudhirkumar Yanpallewar, Deqiang Jing, Colleen A. Barrick, Jodi Becker, Hannah Buckley, Susan G. Dorsey, Francis S. Lee, Lino Tessarollo

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Pathological or in vitro overexpression of the truncated TrkB (TrkB.T1) receptor inhibits signaling through the full-length TrkB (Trk-B.FL) tyrosine kinase receptor. However, to date, the role of endogenous TrkB.T1 is still unknown. By studying mice lacking the truncated TrkB.T1 isoform but retaining normal spatiotemporal expression of TrkB.FL, we have analyzed TrkB.T1-specific physiological functions and its effect on endogenous TrkB kinase signaling in vivo. We found that TrkB.T1-deficient mice develop normally but show increased anxiety in association with morphological abnormalities in the length and complexity of neurites of neurons in the basolateral amygdala. However, no behavioral abnormalities were detected in hippocampal-dependent memory tasks, which correlated with lack of any obvious hippocampal morphological deficits or alterations in basal synaptic transmission and long-term potentiation. In vivo reduction of TrkB signaling by removal of one BDNF allele could be partially rescued by TrkB.T1 deletion, which was revealed by an amelioration of the enhanced aggression and weight gain associated with BDNF haploinsufficiency. Our results suggest that, at the physiological level, TrkB.T1 receptors are important regulators of TrkB.FL signaling in vivo. Moreover, TrkB.T1 selectively affects dendrite complexity of certain neuronal populations.

Original languageEnglish (US)
Pages (from-to)678-685
Number of pages8
JournalJournal of Neuroscience
Volume29
Issue number3
DOIs
StatePublished - Jan 21 2009

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Brain-Derived Neurotrophic Factor
Phosphotransferases
Haploinsufficiency
Long-Term Potentiation
Receptor Protein-Tyrosine Kinases
Neurites
Dendrites
Aggression
Synaptic Transmission
Weight Gain
Protein Isoforms
Anxiety
Alleles
Neurons
Population
Basolateral Nuclear Complex
In Vitro Techniques

Keywords

  • Amygdala
  • Anxiety
  • BDNF
  • Mouse
  • Neurites
  • TrkB.T1

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Carim Todd, L., Bath, K. G., Fulgenzi, G., Yanpallewar, S., Jing, D., Barrick, C. A., ... Tessarollo, L. (2009). Endogenous truncated TrkB.T1 receptor regulates neuronal complexity and TrkB kinase receptor function in vivo. Journal of Neuroscience, 29(3), 678-685. https://doi.org/10.1523/JNEUROSCI.5060-08.2009

Endogenous truncated TrkB.T1 receptor regulates neuronal complexity and TrkB kinase receptor function in vivo. / Carim Todd, Laura; Bath, Kevin G.; Fulgenzi, Gianluca; Yanpallewar, Sudhirkumar; Jing, Deqiang; Barrick, Colleen A.; Becker, Jodi; Buckley, Hannah; Dorsey, Susan G.; Lee, Francis S.; Tessarollo, Lino.

In: Journal of Neuroscience, Vol. 29, No. 3, 21.01.2009, p. 678-685.

Research output: Contribution to journalArticle

Carim Todd, L, Bath, KG, Fulgenzi, G, Yanpallewar, S, Jing, D, Barrick, CA, Becker, J, Buckley, H, Dorsey, SG, Lee, FS & Tessarollo, L 2009, 'Endogenous truncated TrkB.T1 receptor regulates neuronal complexity and TrkB kinase receptor function in vivo', Journal of Neuroscience, vol. 29, no. 3, pp. 678-685. https://doi.org/10.1523/JNEUROSCI.5060-08.2009
Carim Todd, Laura ; Bath, Kevin G. ; Fulgenzi, Gianluca ; Yanpallewar, Sudhirkumar ; Jing, Deqiang ; Barrick, Colleen A. ; Becker, Jodi ; Buckley, Hannah ; Dorsey, Susan G. ; Lee, Francis S. ; Tessarollo, Lino. / Endogenous truncated TrkB.T1 receptor regulates neuronal complexity and TrkB kinase receptor function in vivo. In: Journal of Neuroscience. 2009 ; Vol. 29, No. 3. pp. 678-685.
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