Endocrine precursor cells from mouse islets are not generated by epithelial-to-mesenchymal transition of mature beta cells

Russell A. Morton, Elizabeth Geras-Raaka, Leah M. Wilson, Bruce M. Raaka, Marvin C. Gershengorn

Research output: Contribution to journalArticlepeer-review

Abstract

We previously presented evidence that proliferative human islet precursor cells may be derived in vitro from adult islets by epithelial-to-mesenchymal transition (EMT) and show here that similar fibroblast-like cells can be derived from mouse islets. These mouse cell populations exhibited changes in gene expression consistent with EMT. Both C-peptide and insulin mRNAs were undetectable in expanded cultures of mouse islet-derived precursor cells (mIPCs). After expansion, mIPCs could be induced to migrate into clusters and differentiate into hormone-expressing islet-like aggregates. Although early morphological changes suggesting EMT were observed by time-lapse microscopy when green fluorescent protein-labeled beta cells were placed in culture, the expanded precursor cell population was not fluorescent. Using two mouse models in which beta cells were permanently made either to express alkaline phosphatase or to have a deleted M3 muscarinic receptor, we provide evidence that mIPCs in long term culture are not derived from beta cells.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume270
Issue number1-2
DOIs
StatePublished - May 1 2007
Externally publishedYes

Keywords

  • Beta cells
  • Epithelial-to-mesenchymal transition
  • Genetically marked beta cells
  • Human islet-derived precursor cells
  • Insulin
  • Islet hormones
  • Mouse islet-derived precursor cells
  • Transgenic mouse models

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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