Encapsulated three-dimensional culture supports development of nonhuman primate secondary follicles

Min Xu, Erin R. West-Farrell, Richard Stouffer, Lonnie D. Shea, Teresa K. Woodruff, Mary Zelinski

    Research output: Contribution to journalArticle

    123 Citations (Scopus)

    Abstract

    In vitro ovarian follicle cultures may provide fertilitypreserving options to women facing premature infertility due to cancer therapies. An encapsulated three-dimensional (3-D) culture system utilizing biomaterials to maintain cell-cell communication and support follicle development to produce a mature oocyte has been developed for the mouse. We tested whether this encapsulated 3-D system would also support development of nonhuman primate preantral follicles, for which in vitro growth has not been reported. Three questions were investigated: Does the cycle stage at which the follicles are isolated affect follicle development? Does the rigidity of the hydrogel influence follicle survival and growth? Do follicles require luteinizing hormone (LH), in addition to folliclestimulating hormone (FSH), for steroidogenesis? Secondary follicles were isolated from adult rhesus monkeys, encapsulated within alginate hydrogels, and cultured individually for ≤30 days. Follicles isolated from the follicular phase of the menstrual cycle had a higher survival rate (P <0.05) than those isolated from the luteal phase; however, this difference may also be attributed to differing sizes of follicles isolated during the different stages. Follicles survived and grew in two hydrogel conditions (0.5% and 0.25% alginate). Follicle diameters increased to a greater extent (P <0.05) in the presence of FSH alone than in FSH plus LH. Regardless of gonadotropin treatment, follicles produced estradiol, androstenedione, and progesterone by 14-30 days in vitro. Thus, an alginate hydrogel maintains the 3-D structure of individual secondary macaque follicles, permits follicle growth, and supports steroidogenesis for ≤30 days in vitro. This study documents the first use of the alginate system to maintain primate tissue architecture, and findings suggest that encapsulated 3-D culture will be successful in supporting the in vitro development of human follicles.

    Original languageEnglish (US)
    Pages (from-to)587-594
    Number of pages8
    JournalBiology of Reproduction
    Volume81
    Issue number3
    DOIs
    StatePublished - Sep 2009

    Fingerprint

    Primates
    Hydrogel
    Hormones
    Luteinizing Hormone
    Growth
    Hydrogels
    Ovarian Follicle
    Follicular Phase
    Androstenedione
    Luteal Phase
    Macaca
    Human Development
    Biocompatible Materials
    Macaca mulatta
    Gonadotropins
    Cell Communication
    Infertility
    Oocytes
    Progesterone
    Estradiol

    Keywords

    • Follicle
    • Follicle-stimulating hormone
    • Follicular development
    • Luteinizing hormone
    • Menstrual cycle

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Encapsulated three-dimensional culture supports development of nonhuman primate secondary follicles. / Xu, Min; West-Farrell, Erin R.; Stouffer, Richard; Shea, Lonnie D.; Woodruff, Teresa K.; Zelinski, Mary.

    In: Biology of Reproduction, Vol. 81, No. 3, 09.2009, p. 587-594.

    Research output: Contribution to journalArticle

    Xu, Min ; West-Farrell, Erin R. ; Stouffer, Richard ; Shea, Lonnie D. ; Woodruff, Teresa K. ; Zelinski, Mary. / Encapsulated three-dimensional culture supports development of nonhuman primate secondary follicles. In: Biology of Reproduction. 2009 ; Vol. 81, No. 3. pp. 587-594.
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