Enantioselective measurement of fungal D-arabinitol in the sera of normal adults and patients with candidiasis

Brian Wong, K. L. Brauer

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A new method was used to measure D-arabinitol enantioselectively in the sera of 27 healthy adults and four patients with candidiasis. Arabinitol was measured by gas chromatography in serum that was treated with and without the Klebsiella pneumoniae enzyme D-arabinitol dehydrogenase, lactic dehydrogenase, NAD, and sodium pyruvate. Since enzyme treatment removed 98% of 0 to 20 μg of D-arabinitol per ml and none of 0 to 20 μg of L-arabinitol per ml from spiked sera, D-arabinitol could be determined from the difference in the treated and untreated samples. The concentrations of D- and L-arabinitol in serum from normal subjects were 0.22 ± 0.052 and 0.16 ± 0.055 μg/ml, respectively, and their D-arabinitol/creatinine and L-arabinitol/creatinine ratios were 0.024 ± 0.0089 and 0.017 ± 0.0053 (all means ± standard deviations). The infected patients all had markedly elevated serum D-arabinitol levels, but their L-arabinitol levels were either normal or proportionately much lower. The excess arabinitol in the sera of individuals with candidiasis is D-arabinitol, and use of enantioselective analytical methods should result in improved ability to diagnose and estimate the severity of candidiasis.

Original languageEnglish (US)
Pages (from-to)1670-1674
Number of pages5
JournalJournal of Clinical Microbiology
Volume26
Issue number9
StatePublished - 1988
Externally publishedYes

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Candidiasis
Serum
D-arabinitol dehydrogenase
Creatinine
arabitol
Klebsiella pneumoniae
Enzymes
Pyruvic Acid
Gas Chromatography
NAD
Oxidoreductases
Milk
Sodium

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Enantioselective measurement of fungal D-arabinitol in the sera of normal adults and patients with candidiasis. / Wong, Brian; Brauer, K. L.

In: Journal of Clinical Microbiology, Vol. 26, No. 9, 1988, p. 1670-1674.

Research output: Contribution to journalArticle

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