Embryonic stem cells can be used to construct hybrid cell lines containing a single, selectable murine chromosome

Petra M. Jakobs, Lesley Smith, Mathew Thayer, Markus Grompe

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Microcell-mediated chromosome transfer is a useful technique for the study of gene function, gene regulation, gene mapping, and functional cloning in mammalian cells. Complete panels of donor cell lines, each containing a different human chromosome, have been developed. These donor cell lines contain a single human chromosome marked with a dominant selectable gene in a rodent cell background. However, a similar panel does not exist for murine chromosomes. To produce mouse monochromosomal donor hybrids, we have utilized embryonic stem (ES) cells with targeted gene disruptions of known chromosomal location as starting material. ES cells with mutations in aprt, fyn, and myc were utilized to generate monochromosomal hybrids with neomycin phosphotransferase-marked murine Chr 8, 10, or 15 respectively in a hamster or rat background. This same methodology can be used to generate a complete panel of marked mouse chromosomes for somatic cell genetic experimentation.

Original languageEnglish (US)
Pages (from-to)381-384
Number of pages4
JournalMammalian Genome
Volume10
Issue number4
DOIs
StatePublished - Aug 26 1999

ASJC Scopus subject areas

  • Genetics

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