TY - JOUR
T1 - Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T oncogene
AU - Lindsay Williams, R.
AU - Courtneidge, Sara A.
AU - Wagner, Erwin F.
N1 - Funding Information:
We would like to thank Dymtri Komitowski for carrying out the histological analysis, Colin Stewart for advice and aid, and Angelika Heber for technical assistance. We also thank Rolf Kemler for the gift of the ESD3 cell line, Walter Eckhart for the PymT plasmid. Dave Stoddart and Shirley Pease for maintaining the mouse colony, and our colleagues at the EMBL for advice and crltical reading of the manuscript. R. L. W. is a recipient of a Royal Society European Science Exchange Fellowship Award.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1988/1/15
Y1 - 1988/1/15
N2 - The effect of the middle T oncogene of polyoma virus was studied in vivo using a replication-defective selectable retrovirus. Injection of virus into newborn and adult mice resulted in the rapid appearance of cavernous hemangiomas. Infection of embryos did not yield transgenic mice; therefore, embryonal stem (ES) cells were used as an alternative system. Several infected ES cell clones were established that constitutively expressed middle T and its associated tyrosine kinase activity. Chimeric embryos obtained by blastocyst injection of individual ES cell clones were specifically arrested at midgestation, when multiple hemangiomas disrupted blood vessel formation. From these tumors endothelial cell lines were established that retained expression of von Willebrand factor yet were tumorigenic in vivo. These results suggest that middle T acts in endothelial cells as a single-step oncogene and that ES cells provide a valuable system for the study of growth control during embryogenesis.
AB - The effect of the middle T oncogene of polyoma virus was studied in vivo using a replication-defective selectable retrovirus. Injection of virus into newborn and adult mice resulted in the rapid appearance of cavernous hemangiomas. Infection of embryos did not yield transgenic mice; therefore, embryonal stem (ES) cells were used as an alternative system. Several infected ES cell clones were established that constitutively expressed middle T and its associated tyrosine kinase activity. Chimeric embryos obtained by blastocyst injection of individual ES cell clones were specifically arrested at midgestation, when multiple hemangiomas disrupted blood vessel formation. From these tumors endothelial cell lines were established that retained expression of von Willebrand factor yet were tumorigenic in vivo. These results suggest that middle T acts in endothelial cells as a single-step oncogene and that ES cells provide a valuable system for the study of growth control during embryogenesis.
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U2 - 10.1016/0092-8674(88)90536-3
DO - 10.1016/0092-8674(88)90536-3
M3 - Article
C2 - 3345558
AN - SCOPUS:0023865385
VL - 52
SP - 121
EP - 131
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -