Embryonic cells depleted of β-catenin remain competent to differentiate into dorsal mesodermal derivatives

Francie H. Chu, Bonnie Afonin, Jean Gustin, Alyssa Bost, Michael Sanchez, Carmen R. Domingo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Disruption of axis specification leads to defects in dorsal tissue patterning and cell movements. Here, we examine how β-catenin coordinately affects gastrulation movements and dorsal mesoderm differentiation. The reduction of β-catenin protein levels by morpholino oligonucleotides complementary to β-catenin mRNA causes a disruption in gastrulation movements. Time-lapse imaging of β-catenin morphants during gastrulation reveals that involution occurs simultaneously around the blastopore in the absence of convergent extension cell movements. Transplantation experiments show that morphant cells grafted from the marginal zone into wild-type hosts differentiate into notochord and muscle. However, wild-type mesoderm cells grafted to the marginal zone of β-catenin morphants do not form dorsal tissues. These data argue that β-catenin is not required for the initial establishment of dorsal mesoderm cell competency, but it is required for the maintenance of that competency. We propose that tissue interactions that occur during convergent extension movements are necessary for maintaining dorsal tissue competency.

Original languageEnglish (US)
Pages (from-to)3007-3019
Number of pages13
JournalDevelopmental Dynamics
Volume236
Issue number11
DOIs
StatePublished - Nov 2007

Fingerprint

Catenins
Gastrulation
Mesoderm
Cell Movement
Time-Lapse Imaging
Notochord
Gastrula
Morpholinos
Transplantation
Maintenance
Muscles
Messenger RNA

Keywords

  • β-catenin
  • Cell differentiation
  • Dorsal mesoderm
  • Gastrulation
  • Xenopus laevis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Embryonic cells depleted of β-catenin remain competent to differentiate into dorsal mesodermal derivatives. / Chu, Francie H.; Afonin, Bonnie; Gustin, Jean; Bost, Alyssa; Sanchez, Michael; Domingo, Carmen R.

In: Developmental Dynamics, Vol. 236, No. 11, 11.2007, p. 3007-3019.

Research output: Contribution to journalArticle

Chu, FH, Afonin, B, Gustin, J, Bost, A, Sanchez, M & Domingo, CR 2007, 'Embryonic cells depleted of β-catenin remain competent to differentiate into dorsal mesodermal derivatives', Developmental Dynamics, vol. 236, no. 11, pp. 3007-3019. https://doi.org/10.1002/dvdy.21326
Chu, Francie H. ; Afonin, Bonnie ; Gustin, Jean ; Bost, Alyssa ; Sanchez, Michael ; Domingo, Carmen R. / Embryonic cells depleted of β-catenin remain competent to differentiate into dorsal mesodermal derivatives. In: Developmental Dynamics. 2007 ; Vol. 236, No. 11. pp. 3007-3019.
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