Embryonic ε and γ globin genes of a prosimian primate (Galago crassicaudatus). Nucleotide and amino acid sequences, developmental regulation and phylogenetic footprints

Danilo A. Tagle, Ben F. Koop, Morris Goodman, Jerry L. Slightom, David L. Hess, Richard T. Jones

Research output: Contribution to journalArticlepeer-review

301 Scopus citations

Abstract

Sequence analysis of ε and γ genes and encoded globins and high-pressure liquid chromatography analysis of globin compositions in blood hemolysates obtained from embryos, fetuses and adults show that the prosimian primate Galago crassicaudatus expresses its ε and γ genes only embryonically. Since rabbit, mouse and galago all have embryonic γ genes but simian primates have fetal γ genes, we conclude that γE evolved into γF in stem-simians. An elevated non-synonymous substitution rate characterizes this transition. The alignment of ε and γ nucleotide sequences and the parsimoniously reconstructed evolutionary history of these sequences identify several anciently conserved cis-regulatory elements (phylogenetic footprints) important for γ expression in primates and also cis-mutations which may have been involved in the recruitment of the γ gene to a fetal program in simian primates.

Original languageEnglish (US)
Pages (from-to)439-455
Number of pages17
JournalJournal of molecular biology
Volume203
Issue number2
DOIs
StatePublished - Sep 20 1988
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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