Embryonic ε and γ globin genes of a prosimian primate (Galago crassicaudatus). Nucleotide and amino acid sequences, developmental regulation and phylogenetic footprints

Danilo A. Tagle, Ben F. Koop, Morris Goodman, Jerry L. Slightom, David Hess, Richard T. Jones

    Research output: Contribution to journalArticle

    291 Citations (Scopus)

    Abstract

    Sequence analysis of ε and γ genes and encoded globins and high-pressure liquid chromatography analysis of globin compositions in blood hemolysates obtained from embryos, fetuses and adults show that the prosimian primate Galago crassicaudatus expresses its ε and γ genes only embryonically. Since rabbit, mouse and galago all have embryonic γ genes but simian primates have fetal γ genes, we conclude that γE evolved into γF in stem-simians. An elevated non-synonymous substitution rate characterizes this transition. The alignment of ε and γ nucleotide sequences and the parsimoniously reconstructed evolutionary history of these sequences identify several anciently conserved cis-regulatory elements (phylogenetic footprints) important for γ expression in primates and also cis-mutations which may have been involved in the recruitment of the γ gene to a fetal program in simian primates.

    Original languageEnglish (US)
    Pages (from-to)439-455
    Number of pages17
    JournalJournal of Molecular Biology
    Volume203
    Issue number2
    DOIs
    StatePublished - Sep 20 1988

    Fingerprint

    Galago
    Strepsirhini
    Globins
    Primates
    Amino Acid Sequence
    Nucleotides
    Genes
    Sequence Analysis
    Fetus
    Embryonic Structures
    High Pressure Liquid Chromatography
    Rabbits
    Mutation

    ASJC Scopus subject areas

    • Virology

    Cite this

    Embryonic ε and γ globin genes of a prosimian primate (Galago crassicaudatus). Nucleotide and amino acid sequences, developmental regulation and phylogenetic footprints. / Tagle, Danilo A.; Koop, Ben F.; Goodman, Morris; Slightom, Jerry L.; Hess, David; Jones, Richard T.

    In: Journal of Molecular Biology, Vol. 203, No. 2, 20.09.1988, p. 439-455.

    Research output: Contribution to journalArticle

    Tagle, Danilo A. ; Koop, Ben F. ; Goodman, Morris ; Slightom, Jerry L. ; Hess, David ; Jones, Richard T. / Embryonic ε and γ globin genes of a prosimian primate (Galago crassicaudatus). Nucleotide and amino acid sequences, developmental regulation and phylogenetic footprints. In: Journal of Molecular Biology. 1988 ; Vol. 203, No. 2. pp. 439-455.
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    AU - Koop, Ben F.

    AU - Goodman, Morris

    AU - Slightom, Jerry L.

    AU - Hess, David

    AU - Jones, Richard T.

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    AB - Sequence analysis of ε and γ genes and encoded globins and high-pressure liquid chromatography analysis of globin compositions in blood hemolysates obtained from embryos, fetuses and adults show that the prosimian primate Galago crassicaudatus expresses its ε and γ genes only embryonically. Since rabbit, mouse and galago all have embryonic γ genes but simian primates have fetal γ genes, we conclude that γE evolved into γF in stem-simians. An elevated non-synonymous substitution rate characterizes this transition. The alignment of ε and γ nucleotide sequences and the parsimoniously reconstructed evolutionary history of these sequences identify several anciently conserved cis-regulatory elements (phylogenetic footprints) important for γ expression in primates and also cis-mutations which may have been involved in the recruitment of the γ gene to a fetal program in simian primates.

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