Elongation Arrest by SecM via a Cascade of Ribosomal RNA Rearrangements

Kakoli Mitra, Christiane Schaffitzel, Felcy Fabiola, Michael Chapman, Nenad Ban, Joachim Frank

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled E. coli ribosome complex. A cascade of RNA rearrangements propagates from the exit tunnel throughout the large subunit, affecting intersubunit bridges and tRNA positions, which in turn reorient small subunit RNA elements. Elongation arrest could result from the inhibition of mRNA·(tRNAs) translocation, E site tRNA egress, and perhaps translation factor activation at the GTPase-associated center. Our study suggests that the specific secondary and tertiary arrangement of ribosomal RNA provides the basis for internal signal transduction within the ribosome. Thus, the ribosome may itself have the ability to regulate its progression through translation by modulating its structure and consequently its receptivity to activation by cofactors.

Original languageEnglish (US)
Pages (from-to)533-543
Number of pages11
JournalMolecular Cell
Volume22
Issue number4
DOIs
StatePublished - May 19 2006
Externally publishedYes

Fingerprint

Ribosomal RNA
Transfer RNA
Ribosomes
RNA
Large Ribosome Subunits
Escherichia coli
Cryoelectron Microscopy
GTP Phosphohydrolases
Signal Transduction
Messenger RNA
Peptides

Keywords

  • RNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Elongation Arrest by SecM via a Cascade of Ribosomal RNA Rearrangements. / Mitra, Kakoli; Schaffitzel, Christiane; Fabiola, Felcy; Chapman, Michael; Ban, Nenad; Frank, Joachim.

In: Molecular Cell, Vol. 22, No. 4, 19.05.2006, p. 533-543.

Research output: Contribution to journalArticle

Mitra, K, Schaffitzel, C, Fabiola, F, Chapman, M, Ban, N & Frank, J 2006, 'Elongation Arrest by SecM via a Cascade of Ribosomal RNA Rearrangements', Molecular Cell, vol. 22, no. 4, pp. 533-543. https://doi.org/10.1016/j.molcel.2006.05.003
Mitra, Kakoli ; Schaffitzel, Christiane ; Fabiola, Felcy ; Chapman, Michael ; Ban, Nenad ; Frank, Joachim. / Elongation Arrest by SecM via a Cascade of Ribosomal RNA Rearrangements. In: Molecular Cell. 2006 ; Vol. 22, No. 4. pp. 533-543.
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