Elf-1 and Stat5 bind to a critical element in a new enhancer of the human interleukin-2 receptor α gene

Patrick Lécine, Michèle Algarté, Pascal Rameil, Carol Beadling, Philipp Bucher, Markus Nabholz, Jean Imbert

Research output: Contribution to journalArticle

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Abstract

The interleukin 2 receptor α-chain (IL-2Rα) gene is a key regulator of lymphocyte proliferation. IL-2Rα is rapidly and potently induced in T cells in response to mitogenic stimuli. Interleukin 2 (IL-2) stimulates IL-2Rα transcription, thereby amplifying expression of its own high-affinity receptor. IL-2Rα transcription is at least in part controlled by two positive regulatory regions, PRRI and PRRII. PRRI is an inducible proximal enhancer, located between nucleotides -276 and -244, which contains NF-κB and SRE/CArG motifs. PRRII is a T-cell-specific enhancer, located between nucleotides -137 and -64, which binds the T-cell-specific Ets protein Elf-1 and HMG-1(Y) proteins. However, none of these proximal regions account for the induction of IL-2Rα transcription by IL-2. To find new regulatory regions of the IL-2Rα gene, 8.5 kb of the 5' end noncoding sequence of the IL-2Rα gene have been sequenced. We identified an 86-nucleotide fragment that is 90% identical to the recently characterized murine IL-2-responsive element (mIL-2rE). This putative human IL-2rE, designated PRRIII, confers IL- 2 responsiveness on a heterologous promoter. PRRIII contains a Stat protein binding site that overlaps with an EBS motif (GASd/EBSd). These are essential for IL-2 inducibility of PRRIII/CAT reporter constructs. IL-2 induced the binding of Stat5a and b proteins to the human GASd element. To confirm the physiological relevance of these findings, we carried out in vivo footprinting experiments which showed that stimulation of IL-2Rα expression correlated with occupancy of the GASd element. Our data demonstrate a major role of the GASd/EBSd element in IL-2Rα regulation and suggest that the T- cell-specific Elf-1 factor can serve as a transcriptional repressor.

Original languageEnglish (US)
Pages (from-to)6829-6840
Number of pages12
JournalMolecular and Cellular Biology
Volume16
Issue number12
StatePublished - 1996
Externally publishedYes

Fingerprint

Interleukin-2 Receptors
Interleukin-2
Genes
T-Lymphocytes
Nucleotides
Nucleic Acid Regulatory Sequences
HMGB1 Protein
Protein Binding
Proteins
Binding Sites
Lymphocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Lécine, P., Algarté, M., Rameil, P., Beadling, C., Bucher, P., Nabholz, M., & Imbert, J. (1996). Elf-1 and Stat5 bind to a critical element in a new enhancer of the human interleukin-2 receptor α gene. Molecular and Cellular Biology, 16(12), 6829-6840.

Elf-1 and Stat5 bind to a critical element in a new enhancer of the human interleukin-2 receptor α gene. / Lécine, Patrick; Algarté, Michèle; Rameil, Pascal; Beadling, Carol; Bucher, Philipp; Nabholz, Markus; Imbert, Jean.

In: Molecular and Cellular Biology, Vol. 16, No. 12, 1996, p. 6829-6840.

Research output: Contribution to journalArticle

Lécine, P, Algarté, M, Rameil, P, Beadling, C, Bucher, P, Nabholz, M & Imbert, J 1996, 'Elf-1 and Stat5 bind to a critical element in a new enhancer of the human interleukin-2 receptor α gene', Molecular and Cellular Biology, vol. 16, no. 12, pp. 6829-6840.
Lécine, Patrick ; Algarté, Michèle ; Rameil, Pascal ; Beadling, Carol ; Bucher, Philipp ; Nabholz, Markus ; Imbert, Jean. / Elf-1 and Stat5 bind to a critical element in a new enhancer of the human interleukin-2 receptor α gene. In: Molecular and Cellular Biology. 1996 ; Vol. 16, No. 12. pp. 6829-6840.
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