Elevated plasma atrial natriuretic peptide levels in diabetic rats: Potential mediator of hyperfiltration

F. V. Ortola, B. J. Ballermann, Sharon Anderson, R. E. Mendez, B. M. Brenner

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

Infusion of atrial natriuretic peptide (ANP) increases the glomerular filtration rate (GFR), and ANP is released from cardiac myocytes in response to extracellular fluid volume expansion. Since diabetes mellitus in associated with glomerular hyperfiltration and volume expansion, we investigated the relationship between ANP and GFR in diabetic rats given insulin to achieve stable moderate hyperglycemia or normoglycemia. At 2 wk after induction of diabetes, moderately hyperglycemic diabetic rats exhibited elevations of plasma ANP levels averaging 281 ± 28 pg/ml vs. 158 ± 15 pg/ml in normoglycemic diabetic rats. In hyperglycemic rats, the GFR was also elevated on average to 2.24 ± 0.28 ml/min as compared with 1.71 ± 0.13 ml/min in normoglycemic diabetic rats. To test further the relationship between ANP and GFR in diabetes, moderately hyperglycemic diabetic rats were infused either with a specific ANP antiserum or with nonimmune serum. The infusion of specific ANP antiserum uniformly reduced the GFR on average from 2.38 ± 0.1 ml/min to 1.60 ± 0.1 ml/min, whereas the infusion of nonimmune serum was without effect. It is concluded that elevated endogenous ANP levels contribute to the hyperfiltration observed in early diabetes in the rat.

Original languageEnglish (US)
Pages (from-to)670-674
Number of pages5
JournalJournal of Clinical Investigation
Volume80
Issue number3
StatePublished - 1987
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor
Glomerular Filtration Rate
Immune Sera
Extracellular Fluid
Serum
Cardiac Myocytes
Hyperglycemia
Diabetes Mellitus
Insulin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Elevated plasma atrial natriuretic peptide levels in diabetic rats : Potential mediator of hyperfiltration. / Ortola, F. V.; Ballermann, B. J.; Anderson, Sharon; Mendez, R. E.; Brenner, B. M.

In: Journal of Clinical Investigation, Vol. 80, No. 3, 1987, p. 670-674.

Research output: Contribution to journalArticle

Ortola, F. V. ; Ballermann, B. J. ; Anderson, Sharon ; Mendez, R. E. ; Brenner, B. M. / Elevated plasma atrial natriuretic peptide levels in diabetic rats : Potential mediator of hyperfiltration. In: Journal of Clinical Investigation. 1987 ; Vol. 80, No. 3. pp. 670-674.
@article{2e3e1314cca3457a82c68d0caf72888e,
title = "Elevated plasma atrial natriuretic peptide levels in diabetic rats: Potential mediator of hyperfiltration",
abstract = "Infusion of atrial natriuretic peptide (ANP) increases the glomerular filtration rate (GFR), and ANP is released from cardiac myocytes in response to extracellular fluid volume expansion. Since diabetes mellitus in associated with glomerular hyperfiltration and volume expansion, we investigated the relationship between ANP and GFR in diabetic rats given insulin to achieve stable moderate hyperglycemia or normoglycemia. At 2 wk after induction of diabetes, moderately hyperglycemic diabetic rats exhibited elevations of plasma ANP levels averaging 281 ± 28 pg/ml vs. 158 ± 15 pg/ml in normoglycemic diabetic rats. In hyperglycemic rats, the GFR was also elevated on average to 2.24 ± 0.28 ml/min as compared with 1.71 ± 0.13 ml/min in normoglycemic diabetic rats. To test further the relationship between ANP and GFR in diabetes, moderately hyperglycemic diabetic rats were infused either with a specific ANP antiserum or with nonimmune serum. The infusion of specific ANP antiserum uniformly reduced the GFR on average from 2.38 ± 0.1 ml/min to 1.60 ± 0.1 ml/min, whereas the infusion of nonimmune serum was without effect. It is concluded that elevated endogenous ANP levels contribute to the hyperfiltration observed in early diabetes in the rat.",
author = "Ortola, {F. V.} and Ballermann, {B. J.} and Sharon Anderson and Mendez, {R. E.} and Brenner, {B. M.}",
year = "1987",
language = "English (US)",
volume = "80",
pages = "670--674",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "3",

}

TY - JOUR

T1 - Elevated plasma atrial natriuretic peptide levels in diabetic rats

T2 - Potential mediator of hyperfiltration

AU - Ortola, F. V.

AU - Ballermann, B. J.

AU - Anderson, Sharon

AU - Mendez, R. E.

AU - Brenner, B. M.

PY - 1987

Y1 - 1987

N2 - Infusion of atrial natriuretic peptide (ANP) increases the glomerular filtration rate (GFR), and ANP is released from cardiac myocytes in response to extracellular fluid volume expansion. Since diabetes mellitus in associated with glomerular hyperfiltration and volume expansion, we investigated the relationship between ANP and GFR in diabetic rats given insulin to achieve stable moderate hyperglycemia or normoglycemia. At 2 wk after induction of diabetes, moderately hyperglycemic diabetic rats exhibited elevations of plasma ANP levels averaging 281 ± 28 pg/ml vs. 158 ± 15 pg/ml in normoglycemic diabetic rats. In hyperglycemic rats, the GFR was also elevated on average to 2.24 ± 0.28 ml/min as compared with 1.71 ± 0.13 ml/min in normoglycemic diabetic rats. To test further the relationship between ANP and GFR in diabetes, moderately hyperglycemic diabetic rats were infused either with a specific ANP antiserum or with nonimmune serum. The infusion of specific ANP antiserum uniformly reduced the GFR on average from 2.38 ± 0.1 ml/min to 1.60 ± 0.1 ml/min, whereas the infusion of nonimmune serum was without effect. It is concluded that elevated endogenous ANP levels contribute to the hyperfiltration observed in early diabetes in the rat.

AB - Infusion of atrial natriuretic peptide (ANP) increases the glomerular filtration rate (GFR), and ANP is released from cardiac myocytes in response to extracellular fluid volume expansion. Since diabetes mellitus in associated with glomerular hyperfiltration and volume expansion, we investigated the relationship between ANP and GFR in diabetic rats given insulin to achieve stable moderate hyperglycemia or normoglycemia. At 2 wk after induction of diabetes, moderately hyperglycemic diabetic rats exhibited elevations of plasma ANP levels averaging 281 ± 28 pg/ml vs. 158 ± 15 pg/ml in normoglycemic diabetic rats. In hyperglycemic rats, the GFR was also elevated on average to 2.24 ± 0.28 ml/min as compared with 1.71 ± 0.13 ml/min in normoglycemic diabetic rats. To test further the relationship between ANP and GFR in diabetes, moderately hyperglycemic diabetic rats were infused either with a specific ANP antiserum or with nonimmune serum. The infusion of specific ANP antiserum uniformly reduced the GFR on average from 2.38 ± 0.1 ml/min to 1.60 ± 0.1 ml/min, whereas the infusion of nonimmune serum was without effect. It is concluded that elevated endogenous ANP levels contribute to the hyperfiltration observed in early diabetes in the rat.

UR - http://www.scopus.com/inward/record.url?scp=0023519539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023519539&partnerID=8YFLogxK

M3 - Article

C2 - 2957390

AN - SCOPUS:0023519539

VL - 80

SP - 670

EP - 674

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 3

ER -