Electrophoretically actuated nanoscale optoentropic transduction mechanisms

Benjamin Sullivan, Dietrich Dehlinger, Sanja Zlatanovic, Sadik Esener, Michael J. Heller

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have shown that DNA assembled fluorescent resonant energy transfer systems oscillate in response to electrophoretic actuation in an acrylamide model of in situ hybridization. Integration of fluorescence over time produced clear peaks in Fourier space with signal to noise ratios far above static levels. Frequency responses were demonstrated between 5 Hz to 10 Hz. Actuation speed was likely limited by the ionic flux under low field strength of the relatively macroscale system. The ability to pull signals out of a high background of autofluorescence has important implications for improving the resolution of FISH, and in particular using oligonucleotide probes for the analysis of early stage genetic transitions from hyperplasia to carcinoma.

Original languageEnglish (US)
Title of host publication2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
Pages209-212
Number of pages4
StatePublished - Dec 8 2006
Externally publishedYes
Event2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings - Boston, MA, United States
Duration: May 7 2006May 11 2006

Publication series

Name2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
Volume2

Other

Other2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
CountryUnited States
CityBoston, MA
Period5/7/065/11/06

Keywords

  • Biosensors
  • Electrophoresis
  • FISH
  • FRET
  • Nanoparticles

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Sullivan, B., Dehlinger, D., Zlatanovic, S., Esener, S., & Heller, M. J. (2006). Electrophoretically actuated nanoscale optoentropic transduction mechanisms. In 2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings (pp. 209-212). (2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings; Vol. 2).