Electron microscopical tracers in the uterine epithelium of the pregnant guinea-pig

Dwight J. Kimberly, Kent L. Thornburg

    Research output: Contribution to journalArticle

    4 Scopus citations

    Abstract

    Horseradish peroxidase and lanthanum nitrate were used in pregnant guinea-pigs as electron dense tracers to determine whether the 'permeability' characteristics of the uterine epithelium support the hypothesis that immunoglobulin G gains access to the uterine lumen by transepithelial diffusion. Horseradish peroxidase was injected intravenously in eight animals in experiments ranging from 1-43 min and directly into the uterine lumen in five animals in experiments of 1-8 min duration. Lanthanum nitrate was injected only into the uterine lumen of eight animals for exposures of 1-8 min. Horseradish peroxidase did not traverse the junctional complexes regardless of injection site; lanthanum nitrate did not penetrate the complexes either except in one animal. We conclude that the uterine epithelium is a barrier that prevents the diffusional transfer of IgG from mother to fetus. Further studies are required to locate the site where maternal IgG is transferred to the uterine lumen.

    Original languageEnglish (US)
    Pages (from-to)531-541
    Number of pages11
    JournalPlacenta
    Volume10
    Issue number6
    DOIs
    StatePublished - Jan 1 1989

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Obstetrics and Gynecology
    • Developmental Biology

    Fingerprint Dive into the research topics of 'Electron microscopical tracers in the uterine epithelium of the pregnant guinea-pig'. Together they form a unique fingerprint.

  • Cite this